CJC-1295 DAC (5mg) Research Peptide: A Long‑Acting GHRH Analog for Sustained GH & IGF‑1 Studies

If your research investigates prolonged growth hormone (GH) stimulation, the pharmacokinetics of long‑acting peptide analogues, or the sustained elevation of insulin‑like growth factor 1 (IGF‑1), CJC‑1295 DAC (5mg) research peptide offers a precisely defined synthetic tool with a distinct pharmacological profile. CJC‑1295 DAC is a long‑acting analogue of growth hormone‑releasing hormone (GHRH), engineered to overcome the rapid degradation that limits the utility of native GHRH and shorter‑acting analogues.

The defining feature of CJC-1295 DAC (5mg) is the Drug Affinity Complex (DAC) — a reactive maleimidopropionic acid group that covalently binds to serum albumin, extending the peptide’s half‑life to approximately 6‑8 days in humans. This sustained activity allows researchers to study the effects of prolonged GHRH receptor engagement on GH synthesis and secretion, making CJC‑1295 DAC (5mg) research peptide a valuable tool for laboratories investigating endocrine signalling, metabolic regulation, and the long‑term effects of GH axis modulation.

At Wuhan Top Miracle Peptides, we supply high‑purity CJC‑1295 DAC (5mg) research peptide to research institutions and wholesale buyers across the United Kingdom, France, Germany, Spain, Sweden, Italy, and the USA. Every batch is lyophilised, HPLC‑verified to ≥98% purity, and accompanied by a Certificate of Analysis (COA), ensuring your investigations into sustained GH stimulation and IGF‑1 signalling begin with uncompromising quality.

What Is CJC‑1295 DAC and How Does It Work?

To fully appreciate the research potential of CJC‑1295 DAC (5mg) research peptide, it is essential to understand its biochemical structure and the mechanism by which it achieves sustained activity.

The 30‑Amino‑Acid GHRH Analogue with DAC

CJC‑1295 DAC is a synthetic 30‑amino‑acid peptide derived from GHRH(1‑29), the shortest fully functional fragment of growth hormone‑releasing hormone. The base peptide features four amino acid substitutions that confer resistance to enzymatic degradation: D‑Alanine at position 2 (to prevent DPP‑IV cleavage), Glutamine at position 8 (to reduce asparagine rearrangement), Alanine at position 15 (to enhance bioactivity), and Leucine at position 27 (to prevent methionine oxidation).

The critical distinction is the addition of the Drug Affinity Complex (DAC) — a reactive maleimidopropionic acid group attached to a lysine residue at the C‑terminus. This DAC moiety allows the peptide to covalently bind to serum albumin, dramatically extending its half‑life. Its molecular formula is C₁₆₅H₂₆₉N₄₇O₄₆, with a molecular weight of approximately 3647.15 g/mol. It has a CAS number of 863288‑34‑0.

Mechanism of Action: Sustained GHRH Receptor Activation

The biological activity of CJC‑1295 DAC (5mg) research peptide is mediated through its sustained activation of the GHRH receptor:

• Covalent Binding to Serum Albumin: The DAC moiety reacts selectively with the free thiol group of cysteine‑34 on serum albumin, forming a stable covalent conjugate. This conjugation protects the peptide from renal filtration and proteolytic clearance, allowing it to circulate with albumin’s pharmacokinetic profile. The estimated half‑life of CJC‑1295 is 5.8‑8.1 days.

• Sustained GHRH Receptor Engagement: The albumin‑conjugated peptide binds to GHRH receptors (GHRH‑R) on pituitary somatotrophs. The GHRH‑R is a G‑protein‑coupled receptor (GPCR) that, upon activation, triggers a well‑defined intracellular signalling cascade.

• cAMP/PKA Signalling Cascade: Activation of the Gs protein by the CJC‑1295 DAC‑bound GHRH‑R stimulates adenylyl cyclase to convert ATP into cyclic AMP (cAMP). The increase in intracellular cAMP activates Protein Kinase A (PKA), which phosphorylates downstream targets including transcription factors and ion channels.

• Sustained GH Synthesis and Secretion: This signalling cascade culminates in the synthesis and secretion of growth hormone (GH) from the anterior pituitary. After multiple CJC-1295 DAC (5mg) doses, mean IGF‑I levels remained above baseline for up to 28 days.

Research Note: DAC vs. No‑DAC Distinction

The presence of the DAC moiety fundamentally distinguishes CJC-1295  from its no‑DAC counterpart (also known as Modified GRF 1‑29). While CJC‑1295 without DAC is a short‑acting secretagogue that supports a pulsatile release pattern, CJC‑1295 DAC provides sustained, continuous GHRH receptor engagement. This difference in pharmacokinetic profile leads to distinct research applications: the DAC version is used to study prolonged GH stimulation and sustained IGF‑1 elevation, whereas the no‑DAC version is used to model pulsatile GH release.

Key Research Applications for CJC-1295 DAC (5mg)

CJC‑1295 DAC (5mg) research peptide is a versatile tool with applications spanning several domains of biomedical research.

1. Sustained GH & IGF‑1 Signalling Research

The defining feature of CJC‑1295 DAC—its extended half‑life—makes it a valuable tool for studying prolonged GH and IGF‑1 elevation. Research applications include:

• Pharmacokinetic Studies: Investigating the pharmacokinetic‑like behaviour of peptide‑albumin conjugates and the impact of DAC modification on peptide stability.

• Sustained Signalling Models: Studying the effects of prolonged GHRH receptor engagement on GH synthesis, secretion, and downstream IGF‑1 production.

• IGF‑1 Elevation Studies: Research has shown that mean IGF‑I levels remained above baseline for up to 28 days after multiple CJC‑1295 doses.

2. Metabolic & Body Composition Research

CJC‑1295 DAC has been investigated for its effects on metabolism and body composition:

• Lipolysis and Fat Metabolism: The peptide was originally developed to treat visceral fat deposits, as increased levels of GH are presumed to increase lipolysis (fat loss).

• Muscle Growth and Recovery: Growth hormone secretagogues like CJC‑1295 activate IGF‑1 signalling and satellite cell repair.

• Metabolic Regulation: Studying the long‑term metabolic effects of sustained GH stimulation.

3. Receptor Pharmacology & Signal Transduction Research

As a long‑acting GHRH analogue, CJC‑1295 DAC serves as a molecular probe for studying GHRH receptor pharmacology:

• Receptor Binding Studies: Characterising the binding affinity and selectivity of CJC‑1295 DAC for GHRH‑R.

• Signal Transduction: Dissecting the cAMP/PKA signalling cascade activated by sustained GHRH receptor engagement.

• Receptor Desensitisation: Investigating the potential for GHRH receptor down‑regulation following prolonged exposure.

4. Neuroendocrine & Endocrine Research

CJC‑1295 DAC is used to study the neuroendocrine regulation of the GH axis:

• Hypothalamic‑Pituitary Axis: Investigating the feedback mechanisms that regulate GH secretion, including somatostatin and IGF‑1 negative feedback loops.

• Circadian GH Rhythm: Studying how sustained GHRH stimulation affects the natural circadian rhythm of GH secretion.

5. Orthopaedic & Sports Medicine Research

CJC‑1295 DAC has been evaluated in the context of orthopaedic and sports medicine research:

• Muscle Preservation: CJC‑1295 combined with ipamorelin showed significantly improved maximum tetanic tension in murine models with glucocorticoid‑induced muscle loss.

• Recovery Enhancement: Growth hormone secretagogues are investigated for their potential to support recovery and tissue repair.

Chemical & Physical Specifications for CJC-1295 DAC (5mg)

Accurate characterisation of CJC-1295 DAC (5mg) research peptide is essential for reproducible experimental design.

Note: The 5 mg mass refers to the weight of the peptide in its lyophilised form. CJC‑1295 DAC is typically supplied as a TFA or acetate salt, which enhances aqueous solubility. The peptide contains a DAC moiety that enables covalent binding to serum albumin—a key feature for research applications investigating sustained activity.

Reconstitution & Handling Protocol for CJC‑1295 DAC (5mg)

Proper reconstitution of CJC‑1295 DAC (5mg) research peptide is critical for maintaining its structural integrity and biological activity. The DAC variant has been observed to aggregate faster than the no‑DAC version if not stored properly, so careful handling is essential.

Step 1 – Preparation

• Bring the sealed CJC‑1295 DAC vial and your chosen diluent (sterile bacteriostatic water or sterile PBS, pH 5‑7) to room temperature. Condensation can degrade the lyophilised powder.

• Clean your work surface with 70% ethanol and lay out sterile syringes, alcohol wipes and a clean work mat.

Step 2 – Vial Sanitisation

• Wipe the rubber stopper of the CJC‑1295 DAC vial with a fresh 70% ethanol wipe. Allow the alcohol to evaporate completely before piercing.

Step 3 – Reconstitution

• Draw your desired volume of diluent into a sterile syringe. A common starting concentration for research applications is 5 mg per 1 mL of diluent, yielding a 5 mg/mL stock solution.

• Insert the needle and slowly inject the diluent against the inner glass wall. Do not spray directly onto the lyophilised powder.

• Gently swirl the vial until the solution is clear and fully dissolved. Do not shake vigorously, as shaking can cause denaturation.

Step 4 – Aliquoting & Storage

• Immediately after dissolution, aliquot the reconstituted CJC‑1295 DAC into single‑use sterile vials.

• Store aliquots at –20°C. Avoid repeated freeze‑thaw cycles, as peptides are susceptible to degradation.

• Once thawed, store at 2‑8°C and use within 1‑2 weeks.

Concentration Reference Table for CJC-1295 DAC (5mg)

Adjust diluent volume based on your experimental requirements. For cell culture applications, typical working concentrations may range from 0.1‑10 µM.

Storage & Stability Guidelines for CJC-1295 DAC (5mg)

Why Choose Wuhan Top Miracle Peptides for CJC-1295 DAC (5mg)?

As an authentic licensed peptide supplier based in the United Kingdom, we supply CJC‑1295 DAC (5mg) research peptide and a wide range of research‑grade compounds under rigorous quality assurance protocols, fully aligned with Google’s E‑E‑A‑T standards (Experience, Expertise, Authoritativeness, Trustworthiness).

• Verified Purity: Independent HPLC analysis with purity guaranteed ≥98% for every batch.

• GMP‑Compliant Manufacturing: All peptides are synthesised under strict quality control conditions.

• Full Traceability: Batch‑specific Certificates of Analysis (COA) are available for every order.

• Wholesale Supply: We serve research institutions, laboratories, and wholesale buyers across the UK, EU, and USA with flexible volume pricing and reliable logistics.

• UK‑Based Regulatory Compliance: All products are supplied strictly as research‑grade chemicals for laboratory use only (RUO).

Researchers across the United Kingdom—including those at leading institutions in London, Cambridge, Oxford, Manchester, and Edinburgh—trust us for fast domestic delivery, transparent documentation and professional technical support.

FAQ: CJC-1295 DAC (5mg) Research Peptide

What is CJC-1295 DAC (5mg) research peptide?
CJC‑1295 DAC is a long‑acting synthetic analogue of growth hormone‑releasing hormone (GHRH) that incorporates a Drug Affinity Complex (DAC) moiety. The DAC enables covalent binding to serum albumin, extending the peptide’s half‑life to approximately 6‑8 days. It is used in research to study sustained GH and IGF‑1 stimulation.

What is the sequence and molecular weight of CJC‑1295 DAC?
The sequence is Tyr‑D‑Ala‑Asp‑Ala‑Ile‑Phe‑Thr‑Gln‑Ser‑Tyr‑Arg‑Lys‑Val‑Leu‑Ala‑Gln‑Leu‑Ser‑Ala‑Arg‑Lys‑Leu‑Leu‑Gln‑Asp‑Ile‑Leu‑Ser‑Arg‑LysLys(Maleimidopropionyl)‑NH₂. Its molecular formula is C₁₆₅H₂₆₉N₄₇O₄₆, and its molecular weight is approximately 3647.15 g/mol.

What are the primary research applications for CJC-1295 DAC (5mg)?
CJC‑1295 DAC is used in sustained GH and IGF‑1 signalling research (pharmacokinetic studies, sustained signalling models), metabolic and body composition research (lipolysis, muscle growth), receptor pharmacology and signal transduction studies, neuroendocrine and endocrine research, and orthopaedic and sports medicine research.

What is the mechanism of action of CJC-1295 DAC (5mg)?
CJC-1295 DAC (5mg) binds covalently to serum albumin via its DAC moiety, extending its half‑life. The albumin‑conjugated peptide binds to GHRH receptors on pituitary somatotrophs, activating a Gs‑protein signalling cascade that increases cAMP, activates PKA, and stimulates GH synthesis and secretion. After multiple doses, mean IGF‑I levels remained above baseline for up to 28 days.

How does CJC‑1295 DAC differ from CJC‑1295 without DAC (Mod GRF 1‑29)?
CJC‑1295 with DAC has a half‑life of 6‑8 days and provides sustained, continuous GHRH receptor engagement. CJC‑1295 without DAC (Modified GRF 1‑29) is a short‑acting secretagogue that supports a pulsatile release pattern.

What purity does your CJC‑1295 DAC (5mg) meet?
Our CJC‑1295 DAC is tested to ≥98% purity by HPLC. A Certificate of Analysis (COA) is supplied with every order.

How should I reconstitute CJC‑1295 DAC (5mg)?
Reconstitute with sterile bacteriostatic water or sterile PBS (pH 5‑7). Allow the vial to reach room temperature, then slowly inject the diluent against the inner glass wall and swirl gently. Do not shake. A typical starting concentration is 5 mg/mL.

How should I store reconstituted CJC‑1295 DAC?
Reconstituted CJC‑1295 DAC should be stored at 2‑8°C and used within 1‑2 weeks. For longer storage, aliquot immediately into single‑use vials and store at –20°C for up to 3 months. Avoid repeated freeze‑thaw cycles.

Is CJC‑1295 DAC research peptide legal to purchase in the UK?
Yes. CJC‑1295 DAC is a laboratory reagent sold for research use only (RUO). It is fully legal to purchase, possess, and use in the United Kingdom for legitimate in‑vitro or in‑vivo scientific research, provided it is not marketed with therapeutic claims. Note: CJC‑1295 is not FDA‑approved and is banned by the World Anti‑Doping Agency (WADA) for athletic use.

Does Wuhan Top Miracle Peptides ship internationally?
Yes. We ship across the EU (France, Germany, Spain, Sweden, Italy) and to the USA from our UK warehouse. Wholesale pricing is available for bulk orders.

Wholesale CJC-1295 DAC (5mg) Supply Across the UK and Europe

Wuhan Top Miracle Peptides supports wholesale peptide buyers in the United Kingdom and international markets.

We Support Enquiries From:

• United Kingdom
• France
• Germany
• Spain
• Sweden
• Italy
• USA
• Wider Europe

For larger CJC-1295 DAC 5mg enquiries, contact our team before ordering. This helps confirm stock, documentation, shipping options, DAC status, product identity, and buyer verification requirements.

Where to Source CJC-1295 DAC (5mg) in the UK

If you are searching for where to source CJC-1295 DAC (5mg) in the UK, Wuhan Top Miracle Peptides provides a simple professional enquiry process.

Professional Enquiry Process

1. Review the CJC-1295 DAC (5mg) product details.
2. Check the current label and batch information.
3. Confirm whether the batch is listed asCJC-1295 DAC (5mg) or another verified identity.
4. Confirm DAC status.
5. Ask about COA and purity-related documentation.
6. Confirm the sequence and salt form where required.
7. Confirm storage information and shipping country.
8. Discuss wholesale quantity requirements.
9. Complete buyer verification where required.
10. Place a professional enquiry with support from our team.

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CJC-1295 DAC (5mg)

From its innovative design as a GHRH analogue with a Drug Affinity Complex to its well‑documented role in providing sustained GH stimulation and IGF‑1 elevation, CJC-1295 DAC (5mg) research peptide provides a precisely defined and pharmacologically distinct platform for advanced endocrinological and metabolic research.

Its ability to achieve a half‑life of approximately 6‑8 days through covalent albumin binding—supported by peer‑reviewed research published in the Journal of Clinical Endocrinology & Metabolism and other authoritative journals—makes it a valuable addition to any laboratory investigating sustained GH signalling, IGF‑1 regulation, or the long‑term effects of GHRH receptor engagement. By choosing high‑purity, lab‑verified CJC-1295 DAC (5mg) research peptide from Wuhan Top Miracle Peptides, you ensure that your research is built on a foundation of quality, reproducibility, and scientific integrity.

Ready to advance your research with CJC‑1295 DAC (5mg)? Browse our product catalogue, request a wholesale quote, or contact our support team today to discuss your laboratory’s specific needs. Place your order now and receive GMP‑grade CJC-1295 DAC (5mg) delivered directly to your facility in the UK, EU, or USA.