Pal-GHK (200mg) Research Peptide: A Palmitoylated GHK for Fibroblast & ECM Studies

If your research investigates fibroblast biology, extracellular matrix (ECM) dynamics, or the molecular mechanisms of tissue repair and regeneration, Pal-GHK (200mg) research peptide offers a precisely defined synthetic tool. Also known as Palmitoyl Tripeptide-1 (Pal-GHK), this compound is the palmitoylated (C16 lipid-conjugated) derivative of the well-characterised tripeptide GHK (Gly-His-Lys). The addition of a palmitoyl (C16 fatty acid) chain to the N-terminus of the GHK peptide significantly enhances its lipophilicity and membrane affinity, allowing it to partition into and traverse the stratum corneum lipid bilayers more effectively than the unmodified GHK peptide.

This lipophilic modification transforms the hydrophilic GHK peptide into a more cell-permeable and bioavailable research tool for investigating fibroblast-mediated ECM production, collagen synthesis, and tissue repair signalling pathways. At Wuhan Top Miracle Peptides, we supply high‑purity Pal-GHK (200mg) research peptide to research institutions and wholesale buyers across the United Kingdom, France, Germany, Spain, Sweden, Italy, and the USA. Every batch is lyophilised, HPLC‑verified to ≥98% purity, and accompanied by a Certificate of Analysis (COA), ensuring your investigations into fibroblast biology, ECM remodelling, and tissue repair begin with uncompromising quality.

What Is Pal‑GHK and How Does It Work?

To fully appreciate the research potential of Pal-GHK (200mg) research peptide, it is essential to understand its biochemical design, the basis for its enhanced bioavailability, and the mechanisms by which it modulates cellular behaviour.

The Pal‑GHK Tripeptide: Structure and Design

Pal-GHK is a synthetic tripeptide consisting of glycine, histidine, and lysine (GHK), conjugated at the N‑terminus with a palmitoyl (hexadecanoyl) group. The sequence is Pal‑Gly‑His‑Lys‑OH (Pal‑GHK). Its molecular formula is C₃₀H₅₄N₆O₅, and its molecular weight is approximately 578.8 g/mol. It has a CAS number of 147732-56-7.

The compound is also referred to as Palmitoyl Tripeptide-1, Palmitoyl Oligopeptide, and Biopeptide CL™. The GHK sequence is found naturally in human plasma and is known for its copper-binding properties, which are central to its biological activity. The palmitoyl group—a 16‑carbon saturated fatty acid chain—enhances lipophilicity and membrane affinity, enabling the peptide to interact with lipid bilayers and penetrate cellular membranes more effectively than the unmodified GHK peptide.

Mechanism of Action: Matrikine Signalling and Fibroblast Activation

The biological activity of Pal-GHK (200mg) research peptide is rooted in its function as a signal peptide (matrikine) that mimics fragments of type I collagen. This signalling activates fibroblast-mediated ECM production via TGF‑β‑dependent pathways.

• Matrikine Signalling: Pal-GHK functions as a matrikine—a peptide fragment derived from ECM proteins that acts as a signalling molecule to modulate cellular behaviour. By mimicking collagen fragments, it provides feedback modulation of connective tissue remodelling and proliferative cellular processes.

• TGF‑β Pathway Activation: The peptide is suggested to act via the TGF‑β signalling pathway to stimulate fibrillogenesis and ECM production.

• Fibroblast Proliferation: Pal-GHK promotes human skin fibroblast proliferation in a dose-dependent manner. At concentrations of 0.01, 0.1, and 1 µM, it increased the proliferation rate of human dermal fibroblasts by approximately 20%, 45%, and 60%, respectively, compared to control groups.

• Enhanced Collagen Synthesis: The peptide upregulates the secretion of type I collagen by approximately 55% and type III collagen by approximately 48% in human dermal fibroblasts at 0.1 µM. It has been shown to increase collagen synthesis in human skin fibroblasts in vitro at concentrations as low as 0.5 µM.

• Protection Against Collagen Degradation: Pal-GHK reduces collagen degradation ex vivo in patient‑derived human skin samples irradiated with UVA light when used at a concentration of 6 ppm.

• Skin Barrier Enhancement: The peptide increases the expression of filaggrin and involucrin (skin barrier‑related proteins) by approximately 35‑50% in human keratinocytes at 0.05‑0.5 µM.

• Low Cytotoxicity: Cell viability of human dermal fibroblasts and keratinocytes remained >95% at concentrations up to 10 µM, indicating a favourable safety profile for research applications.

Key Research Applications for Pal‑GHK (200mg)

Pal-GHK (200mg) research peptide is a versatile tool with applications spanning several domains of biomedical research.

1. Fibroblast Biology and ECM Research

The most established application of Pal-GHK lies in its ability to serve as a molecular probe for studying fibroblast function and ECM dynamics. Research applications include:

• Fibroblast Proliferation Studies: Investigating the signalling pathways that regulate fibroblast proliferation and the role of matrikine signalling in tissue homeostasis.

• Collagen Synthesis Assays: Studying the molecular mechanisms underlying type I and type III collagen production in dermal fibroblasts.

• ECM Remodelling: Exploring the balance between ECM synthesis and degradation, including modulation of matrix metalloproteinases (MMPs) and their inhibitors.

• TGF‑β Signalling Pathways: Investigating how palmitoylated peptides activate TGF‑β‑dependent pathways to stimulate fibrillogenesis and ECM production.

2. Tissue Repair and Wound Healing Research

Pal-GHK’s role in promoting fibroblast proliferation and collagen synthesis positions it as a valuable tool for wound healing research:

• Wound Closure Models: Investigating the mechanisms of fibroblast migration and proliferation in wound healing assays.

• Scar Reduction Studies: Exploring how modulation of ECM synthesis and degradation affects scar formation and tissue remodelling.

• Angiogenesis Research: Studying the role of GHK-derived peptides in promoting blood vessel formation during tissue repair.

3. Photoageing and UV Protection Research

Pal-GHK’s ability to reduce collagen degradation in UVA‑irradiated skin samples makes it relevant for photoageing research:

• UV‑Induced Collagen Degradation: Investigating the protective effects of Pal-GHK against UVA‑induced collagen breakdown.

• Oxidative Stress Studies: Exploring the antioxidant properties of GHK‑derived peptides in skin cells.

• Skin Barrier Function: Studying the effects of Pal-GHK on filaggrin and involucrin expression in models of skin barrier disruption.

4. Formulation and Transdermal Delivery Research

The amphiphilic structure of Pal-GHK (200mg) makes it a useful tool for studying peptide delivery systems:

• Lipid‑Based Carriers: Exploring encapsulation in liposomes and solid lipid nanoparticles to enhance peptide delivery.

• Transdermal Penetration Studies: Investigating the role of palmitoylation in enhancing skin permeability and bioavailability.

• Formulation Stability: Studying the stability and activity of Pal-GHK in various formulation contexts.

Chemical & Physical Specifications for Pal-GHK (200mg)

Accurate characterisation of Pal-GHK (200mg) research peptide is essential for reproducible experimental design.

Note: The 200 mg mass refers to the weight of the peptide in its lyophilised form. Pal‑GHK is typically supplied as a TFA or acetate salt, which enhances aqueous solubility. The acetate salt form (CAS 1628252-62-9) has a molecular weight of approximately 636.9 g/mol.

Reconstitution & Handling Protocol for Pal‑GHK (200mg)

Proper reconstitution of Pal-GHK (200mg) research peptide is critical for maintaining its structural integrity and biological activity.

Step 1 – Preparation

• Bring the sealed Pal‑GHK vial and your chosen diluent (sterile bacteriostatic water or sterile PBS, pH 5‑7) to room temperature. Condensation can degrade the lyophilised powder.

• Clean your work surface with 70% ethanol and lay out sterile syringes, alcohol wipes and a clean work mat.

Step 2 – Vial Sanitisation

• Wipe the rubber stopper of the Pal-GHK (200mg) vial with a fresh 70% ethanol wipe. Allow the alcohol to evaporate completely before piercing.

Step 3 – Reconstitution

• Draw your desired volume of diluent into a sterile syringe. A common starting concentration for research applications is 200 mg per 20 mL of diluent, yielding a 10 mg/mL stock solution.

• Insert the needle and slowly inject the diluent against the inner glass wall. Do not spray directly onto the lyophilised powder.

• Gently swirl the vial until the solution is clear and fully dissolved. Do not shake vigorously, as shaking can cause denaturation.

Step 4 – Aliquoting & Storage

• Immediately after dissolution, aliquot the reconstituted Pal‑GHK into single‑use sterile vials.

• Store aliquots at –20°C. Avoid repeated freeze‑thaw cycles.

• Once thawed, store at 2‑8°C and use within 28 days.

Concentration Reference Table for Pal-GHK (200mg)

Adjust diluent volume based on your experimental requirements. For cell culture applications, typical working concentrations range from 0.01‑10 µM.

Storage & Stability Guidelines for Pal-GHK (200mg)

Why Choose Wuhan Top Miracle Peptides for Pal‑GHK (200mg)?

As an authentic licensed peptide supplier based in the United Kingdom, we supply Pal-GHK (200mg) research peptide and a wide range of research‑grade compounds under rigorous quality assurance protocols, fully aligned with Google’s E‑E‑A‑T standards (Experience, Expertise, Authoritativeness, Trustworthiness).

• Verified Purity: Independent HPLC analysis with purity guaranteed ≥98% for every batch.

• GMP‑Compliant Manufacturing: All peptides are synthesised in ISO 13485:2016 and GMP‑certified facilities, ensuring batch‑to‑batch consistency.

• Full Traceability: Batch‑specific Certificates of Analysis (COA) are available for every order.

• Wholesale Supply: We serve research institutions, laboratories, and wholesale buyers across the UK, EU, and USA with flexible volume pricing and reliable logistics.

• UK‑Based Regulatory Compliance: All products are supplied strictly as research‑grade chemicals for laboratory use only (RUO).

Researchers across the United Kingdom—including those at leading institutions in London, Cambridge, Oxford, Manchester, and Edinburgh—trust us for fast domestic delivery, transparent documentation and professional technical support.

FAQ: Pal‑GHK (200mg) Research Peptide

What is Pal‑GHK (200mg) research peptide?
Pal‑GHK (Palmitoyl Tripeptide‑1) is a synthetic tripeptide (Pal‑Gly‑His‑Lys‑OH) conjugated with a palmitoyl (C16 fatty acid) chain. It is the palmitoylated derivative of the naturally occurring GHK peptide, designed to enhance membrane affinity and bioavailability for research applications.

What is the sequence and molecular weight of Pal‑GHK?
The sequence is Pal‑Gly‑His‑Lys‑OH (Pal‑GHK). Its molecular formula is C₃₀H₅₄N₆O₅, and its molecular weight is approximately 578.8 g/mol.

What are the primary research applications for Pal‑GHK?
Pal‑GHK is used in fibroblast biology and ECM research (collagen synthesis, fibroblast proliferation), tissue repair and wound healing studies, photoageing and UV protection research, and formulation and transdermal delivery studies.

What is the mechanism of action of Pal‑GHK?
Pal‑GHK functions as a matrikine (signal peptide) that mimics fragments of type I collagen, activating fibroblast‑mediated ECM production via TGF‑β‑dependent pathways. It promotes fibroblast proliferation, stimulates collagen synthesis, and reduces collagen degradation.

What purity does your Pal‑GHK (200mg) meet?
Our Pal‑GHK is tested to ≥98% purity by HPLC. A Certificate of Analysis (COA) is supplied with every order.

How should I reconstitute Pal‑GHK (200mg)?
Reconstitute with sterile bacteriostatic water or sterile PBS (pH 5‑7). Allow the vial to reach room temperature, then slowly inject the diluent against the inner glass wall and swirl gently. Do not shake. A typical starting concentration is 10 mg/mL.

How should I store reconstituted Pal‑GHK?
Reconstituted Pal‑GHK should be stored at 2‑8°C and used within 28 days. For longer storage, aliquot immediately into single‑use vials and store at –20°C for up to 3 months. Avoid repeated freeze‑thaw cycles.

Is Pal‑GHK research peptide legal to purchase in the UK?
Yes. Pal‑GHK is a laboratory reagent sold for research use only (RUO). It is fully legal to purchase, possess, and use in the United Kingdom for legitimate in‑vitro or in‑vivo scientific research, provided it is not marketed with therapeutic claims.

Does Wuhan Top Miracle Peptides ship internationally?
Yes. We ship across the EU (France, Germany, Spain, Sweden, Italy) and to the USA from our UK warehouse. Wholesale pricing is available for bulk orders.

Wholesale Pal-GHK (200mg) Supply Across the UK and Europe

Wuhan Top Miracle Peptides supports wholesale peptide and cosmetic ingredient buyers in the United Kingdom and international markets.

We Support Enquiries From:

• United Kingdom
• France
• Germany
• Spain
• Sweden
• Italy
• USA
• Wider Europe

For larger Pal-GHK (200mg) enquiries, contact our team before ordering. This helps confirm stock, wholesale pricing, documentation, shipping options, and buyer requirements.

Where to Buy Pal-GHK (200mg) in the UK

If you are searching for where to buy Pal-GHK (200mg) in the UK, Wuhan Top Miracle Peptides provides a simple research-focused enquiry process.

How to Order

1. Review the Pal-GHK (200mg) product details.
2. Check the current label and batch information.
3. Confirm the INCI name or product identity from documentation.
4. Ask about COA and purity-related documentation.
5. Confirm your shipping country.
6. Discuss wholesale quantity requirements.
7. Place your enquiry with support from our team.

 

 

Bulk Peptides Page
Shipping & Returns Page
Contact Us Page
Shop Page
Quality / Compliance Page
Google Search Central
GOV.UK / MHRA
ASA / CAP Code Section 12
Scientific or ingredient reference source such as PubMed, NCBI, PubChem, CosIng, or an official cosmetic ingredient database

From its design as a palmitoylated analogue of the naturally occurring GHK tripeptide to its well‑characterised role in promoting fibroblast proliferation, stimulating collagen synthesis, and modulating ECM dynamics via TGF‑β‑dependent pathways, Pal-GHK (200mg) research peptide provides a precisely defined platform for advanced fibroblast and ECM research. Its ability to enhance membrane affinity and bioavailability through C16 lipidation—supported by in vitro studies demonstrating dose‑dependent fibroblast proliferation and collagen synthesis—makes it a valuable addition to any laboratory investigating fibroblast biology, tissue repair, or ECM remodelling. By choosing high‑purity, lab‑verified Pal-GHK (200mg) research peptide from Wuhan Top Miracle Peptides, you ensure that your research is built on a foundation of quality, reproducibility, and scientific integrity.

Ready to advance your research with Pal‑GHK (200mg)? Browse our product catalogue, request a wholesale quote, or contact our support team today to discuss your laboratory’s specific needs. Place your order now and receive GMP‑grade Pal‑GHK delivered directly to your facility in the UK, EU, or USA.