Cardiogen (20mg) Research Peptide: An AEDR Tetrapeptide for Cardiac & Cellular Repair Studies

If your research investigates cardiac tissue regeneration, fibroblast regulation, or the molecular mechanisms of cellular repair and apoptosis, Cardiogen (20mg) research peptide — also known as the AEDR tetrapeptide (Ala‑Glu‑Asp‑Arg) — offers a precisely defined synthetic tool. Belonging to the Khavinson family of peptide bioregulators, Cardiogen is a short, biologically active tetrapeptide developed from heart tissue-derived polypeptide complexes.

It is classified as a cardiovascular bioregulator peptide and is investigated in preclinical settings for its potential effects on cardiac tissues, fibroblasts, cellular repair mechanisms and apoptosis regulation. Research suggests that Cardiogen may stimulate cardiac cell proliferation, inhibit fibroblast-driven scar formation, and reduce apoptosis by suppressing p53 protein expression. This makes Cardiogen (20mg) research peptide a valuable tool for laboratories investigating cardiovascular biology, tissue regeneration, fibroblast signalling, and the molecular pathways underlying heart function and repair.

At Wuhan Top Miracle Peptides, we supply high‑purity Cardiogen (20mg) research peptide to research institutions and wholesale buyers across the United Kingdom, France, Germany, Spain, Sweden, Italy, and the USA. Every batch is lyophilised, HPLC‑verified to ≥98% purity, and accompanied by a Certificate of Analysis (COA), ensuring your investigations into cardiac biology, fibroblast regulation, and cellular repair begin with uncompromising quality.

What Is Cardiogen and How Does It Work?

To fully appreciate the research potential of Cardiogen (20mg) research peptide, it is essential to understand its biochemical identity and the mechanisms by which it exerts its effects.

The AEDR Tetrapeptide

Cardiogen is a synthetic tetrapeptide consisting of four amino acids: alanine, glutamic acid, aspartic acid, and arginine — the sequence Ala‑Glu‑Asp‑Arg (AEDR). Its molecular formula is C₁₈H₃₁N₇O₉, with a molecular weight of approximately 489.5 g/mol. It has a PubChem CID of 11583989. The peptide belongs to the Khavinson family of short peptide bioregulators — molecules hypothesised to interact with chromatin and DNA to modulate gene expression in a tissue‑specific manner.

Mechanism of Action: Cardiac Repair, Fibroblast Regulation & Gene Modulation

The biological activity of Cardiogen (20mg) research peptide is hypothesised to involve several intersecting mechanisms:

Cardiomyocyte Proliferation and Cardiac Repair

Research suggests Cardiogen stimulates cardiac cell proliferation and promotes heart muscle regeneration. In heart tissue cultures from rats aged 3 months (young) and 24 months (old), Cardiogen uniquely activated cardiomyocyte proliferation while suppressing apoptosis markers like p53 protein — effects not observed with individual amino acids. Notably, Cardiogen’s performance remained robust across both age groups, maintaining its efficacy even in aged tissue where natural regenerative capacity diminishes.

Fibroblast Regulation and Scar Formation Inhibition

Cardiogen targets fibroblasts — key cells in tissue repair — and is hypothesised to inhibit fibroblast-driven scar formation. It may primarily target fibroblasts, which play a crucial role in the formation of scars and tissue regeneration. This dual action — promoting cardiomyocyte proliferation while reducing fibroblast activity — may contribute to reduced scar formation and improved cardiac remodelling.

Apoptosis Regulation and p53 Suppression

Cardiogen reduces apoptosis by suppressing p53 protein expression. The tetrapeptide demonstrated a great stimulating effect on proliferation in myocard tissue cultures from both young and old rats, with an immunohistochemical study demonstrating a decrease of p53 protein expression by Cardiogen action. Researchers consider the peptide to be an apoptotic reductant in cardiac cells via possibly decreasing p53 expression.

DNA Interaction and Gene Expression Modulation

Cardiogen has been shown to modulate DNA hydrolysis and repair processes. It does not bind single‑stranded deoxyribooligonucleotides, slightly quenches fluorescence of some double‑stranded deoxyribooligonucleotides, and strongly quenches fluorescence of methylated and unmethylated λ phage DNA‑ethidium bromide complexes. Its ability to inhibit DNA hydrolysis indicates that it can control endonuclease DNA hydrolysis, and this effect may be mediated through interaction with the enzyme rather than direct binding to DNA.

Key Research Applications for Cardiogen (20mg)

Cardiogen (20mg) research peptide is a versatile tool with applications spanning several domains of biomedical research.

1. Cardiac & Cardiovascular Research

As a cardiovascular bioregulator peptide, Cardiogen is a valuable tool for studying heart function and repair. Research applications include:

• Cardiomyocyte Proliferation: Investigating how Cardiogen stimulates cardiac cell proliferation and regeneration.

• Myocardial Injury Models: Studying Cardiogen’s effects in models of myocardial infarction, heart failure, and ischemia–reperfusion injury.

• Cardiac Remodelling: Exploring how Cardiogen reduces scar formation and improves cardiac remodelling.

• Age‑Related Cardiac Dysfunction: Investigating Cardiogen’s efficacy in aged tissue where natural regenerative capacity diminishes.

2. Fibroblast & Extracellular Matrix Research

As a peptide that targets fibroblasts, Cardiogen is a valuable tool for studying fibroblast biology and ECM dynamics. Research applications include:

• Fibroblast Regulation: Investigating how Cardiogen influences fibroblast activity and scar formation.

• Tissue Repair and Scar Formation: Studying Cardiogen’s effects on fibroblast-driven tissue repair and scar formation.

• Prostate Fibroblast Differentiation: Investigating how Cardiogen enhances the expression of signalling factors involved in fibroblast differentiation in aging prostate cells.

3. Apoptosis & Cell Signalling Research

Cardiogen’s effects on apoptosis and gene expression make it a valuable tool for studying cell signalling and survival pathways:

• p53 Pathway Studies: Investigating how Cardiogen suppresses p53 protein expression and reduces apoptosis.

• Apoptosis Regulation: Exploring Cardiogen’s role as an apoptotic reductant in cardiac cells.

• Gene Expression Modulation: Studying how Cardiogen regulates gene expression and cellular proliferation.

4. Oncology Research

Cardiogen has been investigated for its effects on tumour cells:

• Tumour Apoptosis: Cardiogen may promote tumour cell apoptosis in sarcoma models.

• Tumour Growth Inhibition: In M-1 sarcoma models, Cardiogen induced hemorrhagic necrosis and stimulated tumour cell apoptosis in a dose‑dependent manner.

Chemical & Physical Specifications

Accurate characterisation of Cardiogen (20mg) research peptide is essential for reproducible experimental design.

Reconstitution & Handling Protocol for Cardiogen (20mg)

Proper reconstitution of Cardiogen (20mg) research peptide is critical for maintaining its structural integrity and biological activity.

Step 1 – Preparation

• Bring the sealed Cardiogen vial and your chosen diluent (sterile bacteriostatic water or sterile PBS, pH 5‑7) to room temperature. Condensation can degrade the lyophilised powder.

• Clean your work surface with 70% ethanol and lay out sterile syringes, alcohol wipes and a clean work mat.

Step 2 – Vial Sanitisation

• Wipe the rubber stopper of the Cardiogen vial with a fresh 70% ethanol wipe. Allow the alcohol to evaporate completely before piercing.

Step 3 – Reconstitution

• Draw your desired volume of diluent into a sterile syringe. A common starting concentration for research applications is 20 mg per 2 mL of diluent, yielding a 10 mg/mL stock solution.

• Insert the needle and slowly inject the diluent against the inner glass wall. Do not spray directly onto the lyophilised powder.

• Gently swirl the vial until the solution is clear and fully dissolved. Do not shake vigorously, as shaking can cause denaturation.

Step 4 – Aliquoting & Storage

• Immediately after dissolution, aliquot the reconstituted Cardiogen into single‑use sterile vials.

• Store aliquots at –20°C. Avoid repeated freeze‑thaw cycles, as peptides are susceptible to degradation.

• Once thawed, store at 2‑8°C and use within a short period.

Concentration Reference Table

Adjust diluent volume based on your experimental requirements. For cell culture applications, typical working concentrations may range from 0.1‑10 µM.

Storage & Stability Guidelines

Why Choose Wuhan Top Miracle Peptides for Cardiogen (20mg)?

As an authentic licensed peptide supplier based in the United Kingdom, we supply Cardiogen (20mg) research peptide and a wide range of research‑grade compounds under rigorous quality assurance protocols, fully aligned with Google’s E‑E‑A‑T standards (Experience, Expertise, Authoritativeness, Trustworthiness).

• Verified Purity: Independent HPLC analysis with purity guaranteed ≥98% for every batch.

• GMP‑Compliant Manufacturing: All peptides are synthesised under strict quality control conditions.

• Full Traceability: Batch‑specific Certificates of Analysis (COA) are available for every order.

• Wholesale Supply: We serve research institutions, laboratories, and wholesale buyers across the UK, EU, and USA with flexible volume pricing and reliable logistics.

• UK‑Based Regulatory Compliance: All products are supplied strictly as research‑grade chemicals for laboratory use only (RUO).

Researchers across the United Kingdom—including those at leading institutions in London, Cambridge, Oxford, Manchester, and Edinburgh—trust us for fast domestic delivery, transparent documentation and professional technical support.

FAQ: Cardiogen (20mg) Research Peptide

What is Cardiogen (20mg) research peptide?
Cardiogen is a synthetic tetrapeptide with the sequence Ala‑Glu‑Asp‑Arg (AEDR). It belongs to the Khavinson family of peptide bioregulators and is classified as a cardiovascular bioregulator peptide, investigated in preclinical settings for its effects on cardiac tissues, fibroblasts, cellular repair mechanisms and apoptosis regulation.

What is the sequence and molecular weight of Cardiogen?
The sequence is Ala‑Glu‑Asp‑Arg (AEDR). Its molecular formula is C₁₈H₃₁N₇O₉, and its molecular weight is approximately 489.5 g/mol.

What are the primary research applications for Cardiogen?
Cardiogen is used in cardiac and cardiovascular research (cardiomyocyte proliferation, myocardial injury models, cardiac remodelling), fibroblast and extracellular matrix research, apoptosis and cell signalling research (p53 pathway studies), and oncology research (tumour apoptosis).

 What is the mechanism of action of Cardiogen?
Cardiogen stimulates cardiac cell proliferation, inhibits fibroblast-driven scar formation, and reduces apoptosis by suppressing p53 protein expression. It also modulates DNA hydrolysis and repair processes.

What purity does your Cardiogen (20mg) meet?
Our Cardiogen is tested to ≥98% purity by HPLC. A Certificate of Analysis (COA) is supplied with every order.

How should I reconstitute Cardiogen (20mg)?
Reconstitute with sterile bacteriostatic water or sterile PBS (pH 5‑7). Allow the vial to reach room temperature, then slowly inject the diluent against the inner glass wall and swirl gently. Do not shake. A typical starting concentration is 10 mg/mL.

How should I store reconstituted Cardiogen?
Reconstituted Cardiogen should be stored at 2‑8°C for short‑term use. For longer storage, aliquot immediately into single‑use vials and store at –20°C for up to 3 months. Avoid repeated freeze‑thaw cycles.

Is Cardiogen research peptide legal to purchase in the UK?
Yes. Cardiogen is a laboratory reagent sold for research use only (RUO). It is fully legal to purchase, possess, and use in the United Kingdom for legitimate in‑vitro or in‑vivo scientific research, provided it is not marketed with therapeutic claims. It is not registered as a medicinal product with regulatory agencies such as the MHRA, EMA or FDA.

Does Wuhan Top Miracle Peptides ship internationally?
Yes. We ship across the EU (France, Germany, Spain, Sweden, Italy) and to the USA from our UK warehouse. Wholesale pricing is available for bulk orders.

Wholesale Cardiogen 20mg Supply Across the UK and Europe

Wuhan Top Miracle Peptides supports wholesale peptide buyers in the United Kingdom and international markets.

We Support Enquiries From:

• United Kingdom
• France
• Germany
• Spain
• Sweden
• Italy
• USA
• Wider Europe

For larger Cardiogen 20mg enquiries, contact our team before ordering. This helps confirm stock, documentation, shipping options, product identity, and buyer verification requirements.

Where to Source Cardiogen 20mg in the UK

If you are searching for where to source Cardiogen 20mg in the UK, Wuhan Top Miracle Peptides provides a simple professional enquiry process.

Professional Enquiry Process

1. Review the Cardiogen 20mg product details.
2. Check the current label and batch information.
3. Confirm whether the batch is listed as Cardiogen or another verified identity.
4. Ask about COA and purity-related documentation.
5. Confirm the sequence and salt form where required.
6. Confirm CAS number and storage notes where required.
7. Confirm your shipping country.
8. Discuss wholesale quantity requirements.
9. Complete buyer verification where required.
10. Place a professional enquiry with support from our team.

 

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From its origin as a tetrapeptide developed from heart tissue-derived polypeptide complexes to its well‑characterised role in stimulating cardiomyocyte proliferation, inhibiting fibroblast-driven scar formation, and modulating p53‑mediated apoptosis, Cardiogen (20mg) research peptide provides a precisely defined and increasingly valuable platform for advanced cardiovascular and cellular research.

Its ability to promote cardiac repair, regulate fibroblast activity, and modulate gene expression — supported by peer‑reviewed research published in PubMed and other authoritative journals — makes it a valuable addition to any laboratory investigating cardiac biology, tissue regeneration, fibroblast signalling, or the molecular mechanisms of cellular repair. By choosing high‑purity, lab‑verified Cardiogen (20mg) research peptide from Wuhan Top Miracle Peptides, you ensure that your research is built on a foundation of quality, reproducibility, and scientific integrity.

Ready to advance your research with Cardiogen (20mg)? Browse our product catalogue, request a wholesale quote, or contact our support team today to discuss your laboratory’s specific needs. Place your order now and receive GMP‑grade Cardiogen delivered directly to your facility in the UK, EU, or USA.