PNC-27 (5mg) Research Peptide: A Selective Tool for Oncology Studies

If your research focuses on the molecular mechanisms of cancer cell death, PNC-27 (5mg) research peptide represents a uniquely selective and potent tool. This 32-residue synthetic peptide, designed from the p53 tumour suppressor protein, employs a dual-action mechanism. It binds to the HDM-2 protein on cancer cell membranes and then forms transmembrane pores, leading to rapid cell necrosis and mitochondrial disruption. Critically, PNC-27 is reported to spare healthy cells, potentially due to their distinct membrane composition.

At Wuhan Top Miracle Peptides, we supply high-purity PNC-27 (5mg) research peptide to research institutions across the United Kingdom, France, Germany, Spain, Sweden, Italy, and the USA. This product is intended for laboratory use only. Every batch is lyophilised, HPLC-verified to >98% purity, and comes with a Certificate of Analysis (COA) to ensure your research into novel cancer therapeutics is built on a foundation of uncompromising quality and consistency.

Understanding PNC-27’s Mechanism of Action

The scientific interest in PNC-27 arises from its two distinct but synergistic domains. It combines the HDM-2 binding domain of the p53 protein with a cell-penetrating peptide (CPP) leader sequence. This chimeric design enables a targeted, multi-step process:

• Selective Membrane Binding: PNC-27 binds specifically to the HDM-2 protein expressed on the plasma membrane of cancer cells. This binding is the crucial first step for its cancer-specific activity.

• Transmembrane Pore Formation: Upon binding, the peptide induces the formation of ring-shaped transmembrane pores. These pores cause a rapid influx of extracellular contents and the efflux of vital intracellular components, leading to cancer cell lysis and necrosis.

• Synergy with Paclitaxel: Research suggests PNC-27 may target cancer cells that survive conventional chemotherapy, showing a synergistic effect when used in combination with paclitaxel in ovarian cancer models.

Chemical & Physical Specifications

Key Research Applications for PNC-27 (5mg)

PNC-27 has been extensively researched across several areas of oncology, offering a distinct mechanism for studying cancer cell death.

 Investigating Cancer Cell Necrosis (Poptosis)

PNC-27 is a primary research tool for studying a mechanism of non-apoptotic programmed cell death known as “poptosis.” Unlike chemotherapy, which often induces apoptosis, PNC-27 kills cancer cells by forming plasma membrane pores, leading to direct necrosis—a pathway that may circumvent common drug resistance mechanisms.

 Targeting Therapy-Resistant Cancers

PNC-27 shows significant cytotoxic activity against a range of cancer cell lines, including those resistant to conventional treatments. Preclinical research has demonstrated its efficacy in models of:

• Acute Myeloid Leukaemia (AML): Demonstrated in vivo anti-leukaemic activity.

• Cervical Cancer: Effectively killed cancer cells with no effect on normal cervical cells.

• Colon Cancer: Induced rapid cell death in six different human colon cancer cell lines, without affecting normal colonic fibroblasts.

• Ovarian Cancer: Showed a synergistic effect with paclitaxel, a standard chemotherapy.

 Combinatorial Oncology Studies

Given its distinct mechanism of action, PNC-27 is a valuable research tool in combinatorial oncology protocols. Studies are exploring its ability to target cancer cell populations that survive conventional chemotherapy or radiation, providing a model for investigating the potential to prevent relapse.

Reconstitution & Handling Protocol

Proper reconstitution is critical for the integrity and activity of PNC-27 (5mg) research peptide.

Step 1 – Preparation

• Bring the sealed PNC-27 vial and your chosen diluent (sterile bacteriostatic water or sterile PBS, pH 5–7) to room temperature.

• Clean your work surface with 70% ethanol and lay out sterile syringes, alcohol wipes, and a clean work mat.

Step 2 – Vial Sanitisation

• Wipe the rubber stopper with a fresh 70% ethanol wipe and allow it to evaporate completely.

Step 3 – Reconstitution

• Draw your desired volume of diluent into a sterile syringe. A common starting concentration is 5 mg per 0.5 mL of diluent, yielding a 10 mg/mL stock solution.

• Insert the needle and slowly inject the diluent against the inner glass wall. Do not spray directly onto the lyophilised powder.

• Gently swirl the vial until the solution is clear. Do not shake vigorously, as shaking can cause denaturation.

Step 4 – Aliquoting & Storage

• Immediately aliquot the reconstituted PNC-27 into single-use sterile vials.

• Store aliquots at –20°C, avoid repeated freeze-thaw cycles.

• Once thawed, store at 2-8°C and use within 28 days.

Concentration Reference Table

Formulation Note: For in vitro cell culture research, further dilute the stock solution to the desired working concentration using sterile cell culture media. In vitro studies have used concentrations of 50 μg/mL to induce cell death.

Storage & Stability Guidelines

Why Choose Wuhan Top Miracle Peptides for PNC-27 (5mg)?

As an authentic licensed peptide supplier based in the United Kingdom, we supply PNC-27 (5mg) research peptide under rigorous quality assurance protocols, fully aligned with Google’s E-E-A-T principles.

• Verified Purity: Independent HPLC analysis with purity guaranteed >98% for every batch.

• GMP-Compliant Manufacturing: All peptides are synthesised in ISO 13485:2016 and GMP-certified facilities.

• Full Traceability: Batch-specific Certificates of Analysis (COA) are available for every order.

• Wholesale Supply: Flexible volume pricing for research institutions and laboratories.

• Research Use Only (RUO): All products are supplied strictly as research-grade chemicals for laboratory use only.

FAQ: PNC-27 (5mg) Research Peptide

What is PNC-27 (5mg) research peptide?
PNC-27 is a synthetic 32-residue peptide combining an HDM-2 binding domain from p53 and a cell-penetrating peptide (CPP) leader sequence. It is researched for its selective ability to induce necrosis in cancer cells via transmembrane pore formation.

 What is the mechanism of action?
PNC-27 targets HDM-2 protein on cancer cell membranes, forming pores that cause rapid cell lysis and necrosis. It also enters cells to disrupt mitochondria. Crucially, it spares normal cells, which do not express membrane-bound HDM-2.

 What cancer types is it being researched for?
Research has been conducted on acute myeloid leukaemia (AML), cervical, colon, ovarian, and pancreatic cancers, among others. It has shown activity in both solid and haematopoietic tumours.

 What is the purity level of your PNC-27 (5mg)?
Our PNC-27 research peptide is tested to >98% purity by HPLC. A Certificate of Analysis (COA) is supplied with every order.

How should I reconstitute PNC-27 (5mg)?
Reconstitute with sterile bacteriostatic water or sterile PBS (pH 5–7). Allow the vial to reach room temperature, then slowly inject the diluent against the inner glass wall and swirl gently. Do not shake.

 Is PNC-27 legal to purchase in the UK for research?
Yes. PNC-27 is a laboratory reagent sold for research use only (RUO). It is legal to purchase and use for legitimate in-vitro or in-vivo scientific research, provided it is not marketed for human use.

 What is “poptosis”?
“Poptosis” is a term describing peptide-induced transmembrane pore formation. It is a novel form of cell death used by PNC-27 to target cancer cells while sparing normal ones, acting through a pathway distinct from traditional apoptosis.

 Does Wuhan Top Miracle Peptides ship internationally?
Yes. We ship across the EU (France, Germany, Spain, Sweden, Italy) and to the USA from our UK warehouse. Wholesale pricing is available for bulk orders.

Wholesale PNC-27 5mg Supply Across the UK and Europe

Wuhan Top Miracle Peptides supports wholesale peptide buyers in the United Kingdom and international markets.

We Support Enquiries From:

• United Kingdom
• France
• Germany
• Spain
• Sweden
• Italy
• USA
• Wider Europe

For larger PNC-27 5mg enquiries, contact our team before ordering. This helps confirm stock, wholesale pricing, documentation, shipping options, and buyer requirements.

Where to Buy PNC-27 5mg in the UK

If you are searching for where to buy PNC-27 5mg in the UK, Wuhan Top Miracle Peptides provides a simple research-focused enquiry process.

How to Order

1. Review the PNC-27 5mg product details.
2. Check the current label and batch information.
3. Confirm the product identity from documentation.
4. Contact our team for availability.
5. Ask about COA and purity-related documentation.
6. Confirm your shipping country.
7. Discuss wholesale quantity requirements.
8. Place your enquiry with support from our team.

 

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The unique HDM-2 targeted mechanism of PNC-27 (5mg) research peptide offers a powerful new tool for scientists investigating cancer cell biology. Its ability to induce rapid, selective necrosis through “poptosis” provides a distinct pathway for studying cancer cell death. By choosing high-purity, lab-verified PNC-27 from Wuhan Top Miracle Peptides, you ensure your research is built on a foundation of quality and reproducibility.

Ready to advance your oncology research with PNC-27? Browse our product catalogue, request a wholesale quote, or contact our support team today to discuss your laboratory’s specific needs. Place your order now and receive GMP-grade PNC-27 delivered directly to your facility in the UK, EU, or USA.