Syn-AKE (200mg) Research Peptide: A Synthetic nAChR Antagonist for Neuromuscular Studies

If your research investigates neuromuscular signalling, nicotinic acetylcholine receptor (nAChR) pharmacology, or the molecular mechanisms of muscle contraction, Syn-AKE (200mg) research peptide offers a precisely engineered synthetic tool. Also known as Dipeptide Diaminobutyroyl Benzylamide Diacetate or Tripeptide-3, this compound is a biomimetic tripeptide inspired by the venom of the temple viper (Tropidolaemus wagleri). It mimics the activity of the snake venom peptide waglerin-1, functioning as a reversible antagonist at the muscle nicotinic acetylcholine receptor (mnAChR). By binding to nAChR subunits and blocking acetylcholine from binding, Syn-AKE reduces neuromuscular excitatory signalling.

At Wuhan Top Miracle Peptides, we supply high‑purity Syn-AKE (200mg) research peptide to research institutions and wholesale buyers across the United Kingdom, France, Germany, Spain, Sweden, Italy, and the USA. Every batch is lyophilised, HPLC‑verified to ≥99% purity, and accompanied by a Certificate of Analysis (COA), ensuring your investigations into neuromuscular signalling, receptor pharmacology, and formulation science begin with uncompromising quality.

What Is Syn‑AKE and How Does It Work?

To fully appreciate the research potential of Syn-AKE (200mg) research peptide, it is essential to understand its biochemical design and the precise mechanism by which it modulates neuromuscular signalling.

The Synthetic Tripeptide

Syn‑AKE is a synthetic tripeptide derivative with the sequence β-Ala-Pro-Dab-NH-Bzl (beta-alanyl-L-prolyl-3-aminomethyl-L-alanine benzyl amide). As a diacetate salt, its molecular formula is C₁₉H₂₉N₅O₃·2C₂H₄O₂, and its molecular weight is approximately 495.6 g/mol. It has a CAS number of 823202-99-9.

The compound is also referred to as Tripeptide-3 and is classified as a synthetic peptide derivative and a novel snake venom-like active compound. Its design is inspired by waglerin-1, a 22‑amino‑acid peptide found in the venom of the temple viper (Tropidolaemus wagleri).

Mechanism of Action: Competitive Antagonism at nAChRs

The biological activity of Syn-AKE (200mg) research peptide is rooted in its ability to act as a reversible antagonist at the muscle nicotinic acetylcholine receptor (mnAChR):

• Receptor Binding: Syn‑AKE binds to specific subunits of the nicotinic acetylcholine receptor at the neuromuscular junction, particularly at the interface involving α1 or ε subunits.

• Competitive Inhibition: By occupying the receptor site, it blocks acetylcholine from binding, thereby preventing the receptor from opening its ion channel.

• Reduction of Neuromuscular Signalling: This antagonism reduces neuromuscular excitatory signalling, leading to a relaxation of muscle contractions.

• Reversible Action: Unlike botulinum toxin, which irreversibly cleaves SNARE proteins, Syn‑AKE’s effect is reversible, making it a more controlled tool for research applications.

Binding Affinity and Selectivity

Research indicates that Syn‑AKE acts on the postsynaptic membrane as a reversible antagonist of muscle nicotinic acetylcholine receptors. In vitro studies have demonstrated its ability to bind to the SIRT1 receptor with a binding energy of -9.32 kcal/mol, suggesting stable interactions with key molecular targets.

Key Research Applications for Syn-AKE (200mg)

Syn-AKE (200mg) research peptide is a versatile tool with applications spanning several areas of neuromuscular and formulation research.

Neuromuscular Signalling & nAChR Pharmacology

The most fundamental application of Syn‑AKE lies in its ability to serve as a molecular probe for studying nicotinic acetylcholine receptor function. Its mechanism as a reversible antagonist makes it a valuable tool for:

• Investigating the structural and functional properties of muscle nAChRs

• Studying the kinetics of receptor-ligand interactions at the neuromuscular junction

• Exploring the role of specific nAChR subunits in neuromuscular transmission

• Developing and validating models of receptor antagonism

In vitro studies have shown that Syn‑AKE can reduce muscle contraction frequency by up to 82% within 1 minute in certain experimental models.

 Topical Formulation & Transdermal Research

As a small, synthetic peptide, Syn‑AKE is of significant interest in formulation science. Its physicochemical properties make it suitable for:

• Studying transdermal peptide delivery systems

• Investigating the stability and bioavailability of topical peptide formulations

• Developing and validating novel delivery vehicles for peptide actives

• Exploring the relationship between peptide structure and skin penetration

The compound has been incorporated into various topical vehicles, including creams, serums, and patches, to study formulation variables affecting peptide stability and delivery.

Protein‑Protein Interaction & Molecular Recognition Studies

Beyond its specific role in nAChR pharmacology, Syn‑AKE serves as a model system for studying protein‑peptide interactions. Research applications include:

• Investigating how short peptide fragments mimic the activity of larger venom peptides

• Studying the structural requirements for high‑affinity binding to nAChRs

• Developing computational models of peptide‑receptor interactions

• Exploring the relationship between peptide sequence, conformation, and biological activity

Computational studies have investigated Syn‑AKE’s interactions with matrix metalloproteinases (MMPs) and Sirtuin 1 (SIRT1).

Comparative Neuromuscular Research

Syn‑AKE provides a useful comparator for studying different approaches to neuromuscular modulation. Research applications include:

• Comparing the effects of reversible nAChR antagonists with irreversible SNARE inhibitors (e.g., botulinum toxin)

• Studying the differential effects of various nAChR antagonists on muscle contraction

• Investigating the reversibility and recovery of neuromuscular function after peptide exposure

• Exploring the potential for synergistic effects with other neuromodulatory compounds

Chemical & Physical Specifications

Accurate characterisation of Syn-AKE (200mg) research peptide is essential for reproducible experimental design. Below are the verified specifications from multiple research suppliers.

Note: The 200 mg mass refers to the weight of the peptide in its lyophilised form. Syn‑AKE is typically supplied as a diacetate salt, which enhances aqueous solubility.

Reconstitution & Handling Protocol for Syn-AKE (200mg)

Proper reconstitution of Syn-AKE (200mg) research peptide is critical for maintaining its structural integrity and biological activity.

Step 1 – Preparation

• Bring the sealed Syn‑AKE vial and your chosen diluent (sterile bacteriostatic water or sterile PBS, pH 5‑7) to room temperature. Condensation can degrade the lyophilised powder.

• Clean your work surface with 70% ethanol and lay out sterile syringes, alcohol wipes and a clean work mat.

Step 2 – Vial Sanitisation

• Wipe the rubber stopper of the Syn‑AKE vial with a fresh 70% ethanol wipe. Allow the alcohol to evaporate completely before piercing.

Step 3 – Reconstitution

• Draw your desired volume of diluent into a sterile syringe. A common starting concentration for research applications is 200 mg per 20 mL of diluent, yielding a 10 mg/mL stock solution.

• Insert the needle and slowly inject the diluent against the inner glass wall. Do not spray directly onto the lyophilised powder.

• Gently swirl the vial until the solution is clear and fully dissolved. Do not shake vigorously, as shaking can cause denaturation.

Step 4 – Aliquoting & Storage

• Immediately after dissolution, aliquot the reconstituted Syn‑AKE into single‑use sterile vials.

• Store aliquots at –20°C. Avoid repeated freeze‑thaw cycles.

• Once thawed, store at 2‑8°C and use within 28 days.

Concentration Reference Table

Adjust diluent volume based on your experimental requirements. Syn‑AKE is fully soluble in water at these concentrations.

Storage & Stability Guidelines

Why Choose Wuhan Top Miracle Peptides for Syn-AKE (200mg)?

As an authentic licensed peptide supplier based in the United Kingdom, we supply Syn-AKE (200mg) research peptide and a wide range of research‑grade compounds under rigorous quality assurance protocols, fully aligned with Google’s E‑E‑A‑T standards (Experience, Expertise, Authoritativeness, Trustworthiness).

• Verified Purity: Independent HPLC analysis with purity guaranteed ≥99% for every batch.

• GMP‑Compliant Manufacturing: All peptides are synthesised in ISO 13485:2016 and GMP‑certified facilities, ensuring batch‑to‑batch consistency.

• Full Traceability: Batch‑specific Certificates of Analysis (COA) are available for every order.

• Wholesale Supply: We serve research institutions, laboratories, and wholesale buyers across the UK, EU, and USA with flexible volume pricing and reliable logistics.

• UK‑Based Regulatory Compliance: All products are supplied strictly as research‑grade chemicals for laboratory use only (RUO).

Researchers across the United Kingdom—including those at leading institutions in London, Cambridge, Oxford, Manchester, and Edinburgh—trust us for fast domestic delivery, transparent documentation and professional technical support.

FAQ: Syn-AKE (200mg) Research Peptide

What is Syn-AKE (200mg) research peptide?
Syn‑AKE (Dipeptide Diaminobutyroyl Benzylamide Diacetate, Tripeptide‑3) is a synthetic tripeptide derivative inspired by the venom of the temple viper (Tropidolaemus wagleri). It acts as a reversible antagonist of muscle nicotinic acetylcholine receptors (nAChRs), reducing neuromuscular signalling.

What is the sequence and molecular weight of Syn‑AKE?
The sequence is β‑Ala‑Pro‑Dab‑NH‑Bzl (beta‑alanyl‑L‑prolyl‑3‑aminomethyl‑L‑alanine benzyl amide). Its molecular formula is C₁₉H₂₉N₅O₃·2C₂H₄O₂, and its molecular weight is approximately 495.6 g/mol.

What are the primary research applications for Syn‑AKE?
Syn‑AKE is used in neuromuscular signalling and nAChR pharmacology studies, topical formulation and transdermal research, protein‑protein interaction investigations, and comparative neuromuscular research.

What is the mechanism of action of Syn‑AKE?
Syn‑AKE acts as a reversible antagonist at the muscle nicotinic acetylcholine receptor (mnAChR). It binds to nAChR subunits, blocking acetylcholine from binding and reducing neuromuscular excitatory signalling.

What purity does your Syn-AKE (200mg) meet?
Our Syn‑AKE is tested to ≥99% purity by HPLC. A Certificate of Analysis (COA) is supplied with every order.

How should I reconstitute Syn-AKE (200mg)?
Reconstitute with sterile bacteriostatic water or sterile PBS (pH 5‑7). Allow the vial to reach room temperature, then slowly inject the diluent against the inner glass wall and swirl gently. Do not shake. A typical starting concentration is 10 mg/mL.

How should I store reconstituted Syn‑AKE?
Reconstituted Syn‑AKE should be stored at 2‑8°C and used within 28 days. For longer storage, aliquot immediately into single‑use vials and store at –20°C for up to 3 months. Avoid repeated freeze‑thaw cycles.

Is Syn-AKE research peptide legal to purchase in the UK?
Yes. Syn‑AKE is a laboratory reagent sold for research use only (RUO). It is fully legal to purchase, possess, and use in the United Kingdom for legitimate in‑vitro or in‑vivo scientific research, provided it is not marketed with therapeutic claims.

Does Wuhan Top Miracle Peptides ship internationally?
Yes. We ship across the EU (France, Germany, Spain, Sweden, Italy) and to the USA from our UK warehouse. Wholesale pricing is available for bulk orders.

Wholesale Syn-AKE 200mg Supply Across the UK and Europe

Wuhan Top Miracle Peptides supports wholesale peptide and cosmetic ingredient buyers in the United Kingdom and international markets.

We Support Enquiries From:

• United Kingdom
• France
• Germany
• Spain
• Sweden
• Italy
• USA
• Wider Europe

For larger Syn-AKE 200mg enquiries, contact our team before ordering. This helps confirm stock, wholesale pricing, documentation, shipping options, and buyer requirements.

Where to Buy Syn-AKE 200mg in the UK

If you are searching for where to buy Syn-AKE 200mg in the UK, Wuhan Top Miracle Peptides provides a simple research-focused enquiry process.

How to Order

1. Review the Syn-AKE 200mg product details.
2. Check the current label and batch information.
3. Confirm the INCI name or product identity from documentation.
4. Contact our team for availability.
5. Ask about COA and purity-related documentation.
6. Confirm your shipping country.
7. Discuss wholesale quantity requirements.
8. Place your enquiry with support from our team.

 

 

Bulk Peptides Page
Shipping & Returns Page
Contact Us Page
Shop Page
Quality / Compliance Page
Google Search Central
GOV.UK / MHRA
ASA / CAP Code Section 12
Scientific or ingredient reference source such as PubMed, NCBI, PubChem, CosIng, or an official cosmetic ingredient database

From its design as a biomimetic of the snake venom peptide waglerin‑1 to its well‑characterised role as a reversible antagonist of muscle nicotinic acetylcholine receptors, Syn-AKE (200mg) research peptide provides a precisely defined platform for advanced neuromuscular and formulation research. Its ability to modulate neuromuscular signalling through competitive receptor antagonism—supported by computational studies and in vitro research—makes it a valuable addition to any laboratory investigating nAChR pharmacology, neuromuscular signalling, or topical peptide science. By choosing high‑purity, lab‑verified Syn-AKE (200mg) research peptide from Wuhan Top Miracle Peptides, you ensure that your research is built on a foundation of quality, reproducibility, and scientific integrity.

Ready to advance your research with Syn-AKE (200mg)? Browse our product catalogue, request a wholesale quote, or contact our support team today to discuss your laboratory’s specific needs. Place your order now and receive GMP‑grade Syn‑AKE delivered directly to your facility in the UK, EU, or USA.