Humanin (10mg) Research Peptide: A Mitochondrial-Derived Peptide for Neuroprotection & Aging Studies

If your research investigates mitochondrial biology, neuroprotection, or the molecular mechanisms of aging, Humanin (10mg) research peptide offers a precisely defined and increasingly well-characterised synthetic tool. Humanin is a 24‑amino‑acid peptide encoded within the mitochondrial genome—the first member of a novel class of mitochondrial-derived peptides discovered in over three decades. Initially identified for its ability to protect neurons against Alzheimer’s disease-related neurotoxicity, Humanin has since been shown to exert cytoprotective, anti‑apoptotic, and anti‑inflammatory effects across a diverse range of disease models, including stroke, cardiovascular disease, and cancer. This makes Humanin (10mg) research peptide a valuable tool for laboratories investigating neurodegeneration, mitochondrial dysfunction, metabolic disorders, and the biology of aging.

At Wuhan Top Miracle Peptides, we supply high‑purity Humanin (10mg) research peptide to research institutions and wholesale buyers across the United Kingdom, France, Germany, Spain, Sweden, Italy, and the USA. Every batch is lyophilised, HPLC‑verified to ≥98% purity, and accompanied by a Certificate of Analysis (COA), ensuring your investigations into mitochondrial signalling, neuroprotection, and aging begin with uncompromising quality.

What Is Humanin and How Does It Work?

To fully appreciate the research potential of Humanin (10mg) research peptide, it is essential to understand its unique mitochondrial origin and the mechanisms by which it exerts its pleiotropic effects.

The 24‑Amino‑Acid Mitochondrial Peptide

Humanin is a 24‑amino‑acid peptide encoded within the mitochondrial 16S ribosomal RNA gene (MT‑RNR2). Its sequence is Met‑Ala‑Pro‑Arg‑Gly‑Phe‑Ser‑Cys‑Leu‑Leu‑Leu‑Leu‑Thr‑Ser‑Glu‑Ile‑Asp‑Leu‑Pro‑Val‑Lys‑Arg‑Arg‑Ala (MAPRGFSCLLLLTSEIDLPVKRRA). The peptide has a molecular formula of C₁₁₉H₂₀₄N₃₄O₃₂S₂ and a molecular weight of approximately 2687.23 Da. It has a CAS number of 330936‑69‑1.

Humanin is the founding member of a new class of small signalling peptides that originate from the mitochondrial genome—a discovery that challenged the long‑held view that human mitochondrial DNA encodes only 13 essential components of the electron transport chain. Its expression declines with age, coincident with the increased likelihood for the development of Alzheimer’s disease and other age‑related conditions.

Mechanism of Action: Multi‑Target Cytoprotective Signalling

The biological activity of Humanin (10mg) research peptide is mediated through several intersecting pathways:

Anti‑Apoptotic Activity via Bax Inhibition

Humanin’s primary cytoprotective mechanism involves the inhibition of Bax‑dependent apoptosis. It binds to Bax and prevents its translocation from the cytosol to the mitochondria, blocking the conformational change from the inactive to active form. This action suppresses Bax‑dependent apoptosis and has been demonstrated to protect cells from a range of insults, including oxidative stress, serum starvation, and hypoxia.

Binding to IGFBP‑3 and Regulation of IGF‑I Signalling

Humanin binds with high affinity to insulin‑like growth factor‑binding protein 3 (IGFBP‑3). By binding to IGFBP‑3, Humanin interferes with its nuclear translocation—a prerequisite for IGFBP‑3‑induced apoptosis—and counteracts IGFBP‑3‑mediated cell death. This interaction positions Humanin as a new player in the regulation of the GH‑IGF axis.

Neuroprotection via Trimeric Receptor Signalling

Humanin protects neurons from the toxic effects of amyloid beta (Aβ) by improving hippocampal acetylcholine levels and attenuating oxidative stress and associated neurotoxicity. It acts as a ligand for formyl peptide receptor‑like 1 (FPRL1) and 2 (FPRL2), and suppresses c‑Jun N‑terminal kinase (JNK) and ASK/JNK‑mediated neuronal cell death.

Autophagy Induction and Mitochondrial Protection

Humanin is an autophagy inducer that promotes cell survival in the presence of metabolic and oxidative stress. It restores mitochondrial glutathione levels, increases mitobiogenesis, and preserves mitochondrial function and cell viability under stressful and senescence conditions.

Key Research Applications for Humanin (10mg)

Humanin (10mg) research peptide is a versatile tool with applications spanning several domains of biomedical research.

1. Neurodegenerative Disease & Alzheimer’s Research

The most extensively studied application of Humanin lies in its neuroprotective properties. Research has demonstrated:

• Protection Against Aβ Toxicity: Humanin protects neurons from amyloid beta‑induced toxicity and improves cognitive function in animal models of Alzheimer’s disease.

• Neurotransmitter Support: Humanin improves hippocampal acetylcholine levels, which are critical for memory and cognitive function.

• Biomarker Potential: Humanin is regarded as a biomarker of mitochondrial stress, and its levels change with aging and neurodegeneration.

• Neuroprotective Mechanisms: Humanin suppresses JNK‑mediated neuronal cell death and acts through formyl peptide receptors.

2. Aging & Senescence Research

Humanin levels decline with age, and its restoration has been shown to counteract age‑related processes. Research applications include:

• Age‑Related Decline: Investigating how the age‑related decline in Humanin expression contributes to neurodegeneration and metabolic dysfunction.

• Lifespan and Healthspan: Treatment with Humanin in mice increases both lifespan and healthspan.

• Cellular Senescence: Studying Humanin’s role in preserving mitochondrial function and cell viability under senescence conditions.

• Cardiovascular Aging: Exploring Humanin’s potential to reduce inflammation and oxidative stress in age‑related cardiovascular conditions.

3. Mitochondrial Biology & Metabolic Research

As a mitochondrial‑derived peptide, Humanin is a valuable tool for studying mitochondrial function and metabolism:

• Mitochondrial Protection: Investigating how Humanin preserves mitochondrial functional integrity and restores glutathione levels.

• Insulin Sensitivity: Humanin improves overall insulin sensitivity in animal models and may help prevent insulin resistance.

• Metabolic Disorders: Studying Humanin’s role in regulating autophagy pathways for potential applications in metabolic disorders including diabetes and obesity.

• Exercise Research: High‑intensity interval exercise increases Humanin levels in plasma and muscle, positioning it as a potential exercise‑mimetic molecule.

4. Cardiovascular & Ischaemic Research

Humanin has demonstrated protective effects in cardiovascular disease models:

• Ischaemia/Reperfusion Injury: Humanin protects against cognitive impairment due to ischaemia/reperfusion injury.

• Cardioprotection: Humanin improves outcomes in cardiovascular disease models.

• Anti‑Inflammatory Effects: Humanin reduces inflammation and oxidative stress, interfering with the development of cardiovascular conditions.

Chemical & Physical Specifications

Accurate characterisation of Humanin (10mg) research peptide is essential for reproducible experimental design.

Note: The 10 mg mass refers to the weight of the peptide in its lyophilised form. Humanin is typically supplied as a TFA or acetate salt, which enhances aqueous solubility. The peptide contains a cysteine residue, so care should be taken to avoid oxidation.

Reconstitution & Handling Protocol for Humanin (10mg)

Proper reconstitution of Humanin (10mg) research peptide is critical for maintaining its structural integrity and biological activity.

Step 1 – Preparation

• Bring the sealed Humanin vial and your chosen diluent (sterile bacteriostatic water or sterile PBS, pH 5‑7) to room temperature. Condensation can degrade the lyophilised powder.

• Clean your work surface with 70% ethanol and lay out sterile syringes, alcohol wipes and a clean work mat.

Step 2 – Vial Sanitisation

• Wipe the rubber stopper of the Humanin vial with a fresh 70% ethanol wipe. Allow the alcohol to evaporate completely before piercing.

Step 3 – Reconstitution

• Draw your desired volume of diluent into a sterile syringe. A common starting concentration for research applications is 10 mg per 1 mL of diluent, yielding a 10 mg/mL stock solution.

• Insert the needle and slowly inject the diluent against the inner glass wall. Do not spray directly onto the lyophilised powder.

• Gently swirl the vial until the solution is clear and fully dissolved. Do not shake vigorously, as shaking can cause denaturation.

Step 4 – Aliquoting & Storage

• Immediately after dissolution, aliquot the reconstituted Humanin into single‑use sterile vials.

• Store aliquots at –20°C. Avoid repeated freeze‑thaw cycles, as peptides are susceptible to degradation.

• Once thawed, store at 2‑8°C and use within a short period.

Concentration Reference Table

Adjust diluent volume based on your experimental requirements. For cell culture applications, typical working concentrations range from 0.1‑10 µM.

Storage & Stability Guidelines

Why Choose Wuhan Top Miracle Peptides for Humanin (10mg)?

As an authentic licensed peptide supplier based in the United Kingdom, we supply Humanin (10mg) research peptide and a wide range of research‑grade compounds under rigorous quality assurance protocols, fully aligned with Google’s E‑E‑A‑T standards (Experience, Expertise, Authoritativeness, Trustworthiness).

• Verified Purity: Independent HPLC analysis with purity guaranteed ≥98% for every batch.

• GMP‑Compliant Manufacturing: All peptides are synthesised under strict quality control conditions.

• Full Traceability: Batch‑specific Certificates of Analysis (COA) are available for every order.

• Wholesale Supply: We serve research institutions, laboratories, and wholesale buyers across the UK, EU, and USA with flexible volume pricing and reliable logistics.

• UK‑Based Regulatory Compliance: All products are supplied strictly as research‑grade chemicals for laboratory use only (RUO).

Researchers across the United Kingdom—including those at leading institutions in London, Cambridge, Oxford, Manchester, and Edinburgh—trust us for fast domestic delivery, transparent documentation and professional technical support.

FAQ: Humanin (10mg) Research Peptide

What is Humanin (10mg) research peptide?
Humanin is a 24‑amino‑acid peptide encoded within the mitochondrial genome—the first member of a novel class of mitochondrial-derived peptides. It exhibits neuroprotective, cytoprotective, anti‑apoptotic, and anti‑inflammatory properties across a diverse range of disease models.

What is the sequence and molecular weight of Humanin?
The sequence is Met‑Ala‑Pro‑Arg‑Gly‑Phe‑Ser‑Cys‑Leu‑Leu‑Leu‑Leu‑Thr‑Ser‑Glu‑Ile‑Asp‑Leu‑Pro‑Val‑Lys‑Arg‑Arg‑Ala (MAPRGFSCLLLLTSEIDLPVKRRA). Its molecular weight is approximately 2687.23 Da.

What are the primary research applications for Humanin?
Humanin is used in neurodegenerative disease and Alzheimer’s research, aging and senescence studies, mitochondrial biology and metabolic research, and cardiovascular and ischaemic research.

What is the mechanism of action of Humanin?
Humanin acts through multiple mechanisms: it inhibits Bax‑dependent apoptosis, binds to IGFBP‑3 to regulate IGF‑I signalling and prevent IGFBP‑3‑induced cell death, activates neuroprotective signalling through formyl peptide receptors, and induces autophagy to promote cell survival under stress.

What purity does your Humanin (10mg) meet?
Our Humanin is tested to ≥98% purity by HPLC. A Certificate of Analysis (COA) is supplied with every order.

How should I reconstitute Humanin (10mg)?
Reconstitute with sterile bacteriostatic water or sterile PBS (pH 5‑7). Allow the vial to reach room temperature, then slowly inject the diluent against the inner glass wall and swirl gently. Do not shake.

How should I store reconstituted Humanin?
Reconstituted Humanin should be stored at 2‑8°C for short‑term use. For longer storage, aliquot immediately into single‑use vials and store at –20°C for up to 3 months. Avoid repeated freeze‑thaw cycles.

Is Humanin research peptide legal to purchase in the UK?
Yes. Humanin is a laboratory reagent sold for research use only (RUO). It is fully legal to purchase, possess, and use in the United Kingdom for legitimate in‑vitro or in‑vivo scientific research, provided it is not marketed with therapeutic claims.

Does Wuhan Top Miracle Peptides ship internationally?
Yes. We ship across the EU (France, Germany, Spain, Sweden, Italy) and to the USA from our UK warehouse. Wholesale pricing is available for bulk orders.

Wholesale Humanin 10mg Supply Across the UK and Europe

Wuhan Top Miracle Peptides supports wholesale peptide buyers in the United Kingdom and international markets.

We Support Enquiries From:

• United Kingdom
• France
• Germany
• Spain
• Sweden
• Italy
• USA
• Wider Europe

For larger Humanin 10mg enquiries, contact our team before ordering. This helps confirm stock, documentation, shipping options, product identity, and buyer verification requirements.

Where to Source Humanin 10mg in the UK

If you are searching for where to source Humanin 10mg in the UK, Wuhan Top Miracle Peptides provides a simple professional enquiry process.

Professional Enquiry Process

1. Review the Humanin 10mg product details.
2. Check the current label and batch information.
3. Confirm whether the batch is listed as Humanin, HN, MT-RNR2 peptide, or another verified identity.
4. Ask about COA and purity-related documentation.
5. Confirm the sequence and salt form where required.
6. Confirm your shipping country.
7. Discuss wholesale quantity requirements.
8. Complete buyer verification where required.
9. Place a professional enquiry with support from our team.

 

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From its discovery as the first mitochondrial‑encoded signalling peptide to its well‑characterised role in neuroprotection, anti‑apoptosis, and metabolic regulation, Humanin (10mg) research peptide provides a precisely defined and increasingly valuable platform for advanced mitochondrial and biomedical research. Its ability to inhibit Bax‑dependent apoptosis, bind IGFBP‑3 to regulate IGF‑I signalling, induce autophagy, and protect against oxidative stress—supported by a growing body of peer‑reviewed literature published in Cell Metabolism, Nature, and other authoritative journals—makes it a valuable addition to any laboratory investigating neurodegeneration, mitochondrial biology, or the molecular mechanisms of aging. By choosing high‑purity, lab‑verified Humanin (10mg) research peptide from Wuhan Top Miracle Peptides, you ensure that your research is built on a foundation of quality, reproducibility, and scientific integrity.

Ready to advance your research with Humanin (10mg)? Browse our product catalogue, request a wholesale quote, or contact our support team today to discuss your laboratory’s specific needs. Place your order now and receive GMP‑grade Humanin delivered directly to your facility in the UK, EU, or USA.