Kisspeptin-10 (10mg) Research Peptide: A Master Regulator of the HPG Axis & Beyond

If your research investigates reproductive endocrinology, neuroendocrine signalling, or the molecular mechanisms of metastasis suppression, Kisspeptin-10 (10mg) research peptide offers a precisely defined and extensively validated synthetic tool. Kisspeptin-10 (KP-10, Metastin 45-54) is a decapeptide derived from the product of the KISS1 gene and the endogenous ligand for the Kisspeptin receptor (KISS1, GPR54).

It has emerged as a master regulator of the hypothalamic-pituitary-gonadal (HPG) axis and, consequently, a critical component in the neuroendocrine control of reproduction. Beyond its role in reproductive biology, Kisspeptin-10 has demonstrated significant potential in bone metabolism research and as a metastasis suppressor in various cancer models. This makes Kisspeptin-10 (10mg) research peptide a uniquely versatile tool for laboratories investigating endocrinology, neuroscience, oncology, and bone biology.

At Wuhan Top Miracle Peptides, we supply high‑purity Kisspeptin-10 (10mg) research peptide to research institutions and wholesale buyers across the United Kingdom, France, Germany, Spain, Sweden, Italy, and the USA. Every batch is lyophilised, HPLC‑verified to ≥98% purity, and accompanied by a Certificate of Analysis (COA), ensuring your investigations into reproductive endocrinology, neuroendocrine signalling, and metastasis suppression begin with uncompromising quality.

What Is Kisspeptin-10 and How Does It Work?

To fully appreciate the research potential of Kisspeptin-10 (10mg) research peptide, it is essential to understand its biochemical structure and the diverse mechanisms by which it exerts its effects.

The Decapeptide Derived from KISS1

Kisspeptin-10 is a synthetic decapeptide corresponding to the C‑terminal amino acids 45-54 of the Kisspeptin precursor protein (metastin). Its sequence is Tyr‑Asn‑Trp‑Asn‑Ser‑Phe‑Gly‑Leu‑Arg‑Phe‑NH₂ (YNWNSFGLRF-NH₂). The peptide has a molecular formula of C₆₃H₈₃N₁₇O₁₄ and a molecular weight of approximately 1302.5 Da. It has a CAS number of 374675-21-5.

The peptide is the minimal sequence needed to activate GPR54 signalling and shares similar affinities and functions with the longer kisspeptin isoforms. The C‑terminal amidation is essential for its biological activity.

Mechanism of Action: GPR54 Agonism and Downstream Signalling

The biological activity of Kisspeptin-10 (10mg) research peptide is mediated through its potent and selective activation of the Kisspeptin receptor (KISS1, GPR54):

• High‑Affinity Receptor Binding: Kisspeptin-10 binds with high affinity to rat and human KISS1 receptors, with Ki values of 1.59 nM and 2.33 nM, respectively.

• HPG Axis Regulation: Activation of GPR54 in the hypothalamus stimulates the release of luteinising hormone‑releasing hormone (LHRH) from hypothalamic explants. This, in turn, drives the secretion of luteinising hormone (LH) and follicle‑stimulating hormone (FSH) from the pituitary, leading to increased gonadal steroidogenesis. Mutations in the Kiss1 receptor are known to cause hypogonadotropic hypogonadism in humans and mice.

• Direct Pituitary Effects: Kisspeptins also exert direct effects on the pituitary, activating gonadotrophs and somatotrophs and stimulating the secretion of LH and growth hormone.

• Bone Metabolism: GPR54 activation by Kisspeptin-10 in osteoclasts causes Dusp18 to dephosphorylate Src at Tyr 416, a pathway that suppresses osteoclast activity and prevents bone loss.

• Metastasis Suppression: The KISS1 gene was originally discovered as a metastasis suppressor gene. Kisspeptin-10 inhibits tumour angiogenesis by suppressing Sp1‑mediated VEGF expression and FAK/Rho GTPase activation.

Key Research Applications for Kisspeptin-10 (10mg)

Kisspeptin-10 (10mg) research peptide is a versatile tool with applications spanning several domains of biomedical research.

1. Reproductive Endocrinology & HPG Axis Research

As the master regulator of the HPG axis, Kisspeptin-10 is an essential tool for studying the neuroendocrine control of reproduction. Research applications include:

• Puberty and Fertility: Investigating the role of the kisspeptin/GPR54 system in the onset of puberty and the regulation of fertility.

• GnRH Neuroendocrine System: Studying how Kisspeptin-10 stimulates GnRH release through the Kiss1 receptor.

• Gonadotropin Regulation: Exploring the dose‑dependent effects of Kisspeptin-10 on plasma LH, FSH, and testosterone.

• Reproductive Disorders: Investigating potential therapeutic applications for infertility, amenorrhea, and pubertal delay.

2. Bone Metabolism & Osteoclast Research

Kisspeptin-10 has emerged as a critical regulator of bone homeostasis. Research has demonstrated:

• Osteoclast Activity Suppression: Kisspeptin-10 binding to Gpr54 in osteoclasts prevents bone loss by activating Dusp18‑mediated dephosphorylation of Src.

• Therapeutic Target: The Kp‑10/Gpr54 axis is a promising therapeutic target to abrogate bone resorption.

• Age‑Related Bone Loss: Investigating how Kisspeptin-10 signalling may counteract the over‑activation of osteoclasts observed with aging.

3. Oncology & Metastasis Research

The KISS1 gene was first discovered as a metastasis suppressor gene. Kisspeptin-10 is a valuable tool for cancer research:

• Metastasis Suppression: Kisspeptin-10 inhibits metastasis and invasion in mouse melanomas and other cancers including gastric, pancreatic, ovarian, and prostate cancer.

• Tumour Angiogenesis Inhibition: Kisspeptin-10 inhibits angiogenesis by suppressing Sp1‑mediated VEGF expression and FAK/Rho GTPase activation.

• Triple‑Negative Breast Cancer: Exogenous Kisspeptin-10 treatment shows pleiotropic effects, including metastasis suppression and promotion of apoptosis.

• Diagnostic Imaging: Radionuclide‑labelled kisspeptin peptides provide a potential target for diagnostic imaging and potentially pan‑tumour therapeutic radiopharmaceuticals.

4. Neuroendocrinology & Behavioural Research

Emerging research has explored Kisspeptin-10’s effects beyond the HPG axis:

• Antidepressant‑Like Effects: The antidepressant‑like effects of Kisspeptin-10 are reversed by a kisspeptin antagonist, suggesting a role in mood regulation.

• Cerebral Aneurysm Prevention: The Kisspeptin‑10/GPR54 signalling pathway is dysregulated in individuals with cerebral aneurysms, and KP‑10 administration prevents their formation in mouse models.

Chemical & Physical Specifications

Accurate characterisation of Kisspeptin-10 (10mg) research peptide is essential for reproducible experimental design.

Note: The 10 mg mass refers to the weight of the peptide in its lyophilised form. Kisspeptin-10 is typically supplied as a TFA or acetate salt, which enhances aqueous solubility. The C‑terminal amide is essential for biological activity.

Reconstitution & Handling Protocol for Kisspeptin-10 (10mg)

Proper reconstitution of Kisspeptin-10 (10mg) research peptide is critical for maintaining its structural integrity and biological activity. The peptide has a very rapid clearance in vivo, so careful handling is essential.

Step 1 – Preparation

• Bring the sealed Kisspeptin-10 vial and your chosen diluent (sterile bacteriostatic water or sterile PBS, pH 5‑7) to room temperature. Condensation can degrade the lyophilised powder.

• Clean your work surface with 70% ethanol and lay out sterile syringes, alcohol wipes and a clean work mat.

Step 2 – Vial Sanitisation

• Wipe the rubber stopper of the Kisspeptin-10 vial with a fresh 70% ethanol wipe. Allow the alcohol to evaporate completely before piercing.

Step 3 – Reconstitution

• Draw your desired volume of diluent into a sterile syringe. A common starting concentration for research applications is 10 mg per 1 mL of diluent, yielding a 10 mg/mL stock solution. Note that the peptide is soluble in water up to 1 mg/mL; for higher concentrations, gentle sonication may be required.

• Insert the needle and slowly inject the diluent against the inner glass wall. Do not spray directly onto the lyophilised powder.

• Gently swirl the vial until the solution is clear and fully dissolved. Do not shake vigorously, as shaking can cause denaturation.

Step 4 – Aliquoting & Storage

• Immediately after dissolution, aliquot the reconstituted Kisspeptin-10 into single‑use sterile vials.

• Store aliquots at –20°C. Avoid repeated freeze‑thaw cycles, as peptides are susceptible to degradation.

• Once thawed, store at 2‑8°C and use within a short period.

Concentration Reference Table

Adjust diluent volume based on your experimental requirements. For cell culture applications, typical working concentrations range from 0.1‑10 µM.

Storage & Stability Guidelines

Why Choose Wuhan Top Miracle Peptides for Kisspeptin-10 (10mg)?

As an authentic licensed peptide supplier based in the United Kingdom, we supply Kisspeptin-10 (10mg) research peptide and a wide range of research‑grade compounds under rigorous quality assurance protocols, fully aligned with Google’s E‑E‑A‑T standards (Experience, Expertise, Authoritativeness, Trustworthiness).

• Verified Purity: Independent HPLC analysis with purity guaranteed ≥98% for every batch.

• GMP‑Compliant Manufacturing: All peptides are synthesised under strict quality control conditions.

• Full Traceability: Batch‑specific Certificates of Analysis (COA) are available for every order.

• Wholesale Supply: We serve research institutions, laboratories, and wholesale buyers across the UK, EU, and USA with flexible volume pricing and reliable logistics.

• UK‑Based Regulatory Compliance: All products are supplied strictly as research‑grade chemicals for laboratory use only (RUO).

Researchers across the United Kingdom—including those at leading institutions in London, Cambridge, Oxford, Manchester, and Edinburgh—trust us for fast domestic delivery, transparent documentation and professional technical support.

FAQ: Kisspeptin-10 (10mg) Research Peptide

Q1: What is Kisspeptin-10 (10mg) research peptide?
Kisspeptin-10 (KP-10, Metastin 45-54) is a decapeptide derived from the KISS1 gene product and the endogenous ligand for the Kisspeptin receptor (KISS1, GPR54). It is a master regulator of the hypothalamic-pituitary-gonadal (HPG) axis and also functions as a metastasis suppressor and regulator of bone metabolism.

Q2: What is the sequence and molecular weight of Kisspeptin-10?
The sequence is Tyr‑Asn‑Trp‑Asn‑Ser‑Phe‑Gly‑Leu‑Arg‑Phe‑NH₂ (YNWNSFGLRF-NH₂). Its molecular weight is approximately 1302.5 Da.

Q3: What are the primary research applications for Kisspeptin-10?
Kisspeptin-10 is used in reproductive endocrinology and HPG axis research (puberty, fertility, gonadotropin regulation), bone metabolism and osteoclast studies (preventing bone loss), oncology and metastasis research (suppressing metastasis and angiogenesis), and neuroendocrinology and behavioural research (antidepressant effects, cerebral aneurysm prevention).

Q4: What is the mechanism of action of Kisspeptin-10?
Kisspeptin-10 binds with high affinity to the Kisspeptin receptor (GPR54). This activation stimulates the release of LHRH from the hypothalamus, driving LH and FSH secretion. It also exerts direct pituitary effects, suppresses osteoclast activity via Dusp18‑mediated Src dephosphorylation, and inhibits tumour angiogenesis and metastasis.

Q5: What purity does your Kisspeptin-10 (10mg) meet?
Our Kisspeptin-10 is tested to ≥98% purity by HPLC. A Certificate of Analysis (COA) is supplied with every order.

Q6: How should I reconstitute Kisspeptin-10 (10mg)?
Reconstitute with sterile bacteriostatic water or sterile PBS (pH 5‑7). Allow the vial to reach room temperature, then slowly inject the diluent against the inner glass wall and swirl gently. Do not shake. A typical starting concentration is 10 mg/mL.

Q7: How should I store reconstituted Kisspeptin-10?
Reconstituted Kisspeptin-10 should be stored at 2‑8°C for short‑term use. For longer storage, aliquot immediately into single‑use vials and store at –20°C for up to 3 months. Avoid repeated freeze‑thaw cycles.

Q8: Is Kisspeptin-10 research peptide legal to purchase in the UK?
Yes. Kisspeptin-10 is a laboratory reagent sold for research use only (RUO). It is fully legal to purchase, possess, and use in the United Kingdom for legitimate in‑vitro or in‑vivo scientific research, provided it is not marketed with therapeutic claims.

Q9: Does Wuhan Top Miracle Peptides ship internationally?
Yes. We ship across the EU (France, Germany, Spain, Sweden, Italy) and to the USA from our UK warehouse. Wholesale pricing is available for bulk orders.

Wholesale Kisspeptin-10 10mg Supply Across the UK and Europe

Wuhan Top Miracle Peptides supports wholesale peptide buyers in the United Kingdom and international markets.

We Support Enquiries From:

• United Kingdom
• France
• Germany
• Spain
• Sweden
• Italy
• USA
• Wider Europe

For larger Kisspeptin-10 10mg enquiries, contact our team before ordering. This helps confirm stock, documentation, shipping options, product identity, and buyer verification requirements.

Where to Source Kisspeptin-10 10mg in the UK

If you are searching for where to source Kisspeptin-10 10mg in the UK, Wuhan Top Miracle Peptides provides a simple professional enquiry process.

Professional Enquiry Process

1. Review the Kisspeptin-10 10mg product details.
2. Check the current label and batch information.
3. Confirm whether the batch is listed as Kisspeptin-10, Kp-10, or another verified identity.
4. Ask about COA and purity-related documentation.
5. Confirm the sequence, amidation status, and salt form where required.
6. Confirm your shipping country.
7. Discuss wholesale quantity requirements.
8. Complete buyer verification where required.
9. Place a professional enquiry with support from our team.

 

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From its discovery as the endogenous ligand for GPR54 to its well‑characterised role as a master regulator of the HPG axis, a suppressor of metastasis, and a regulator of bone metabolism, Kisspeptin-10 (10mg) research peptide provides a precisely defined and extensively validated platform for advanced endocrinological, oncological, and bone biology research.

Its high‑affinity receptor binding (Ki = 1.59 nM for rat, 2.33 nM for human KISS1 receptors), ability to stimulate the HPG axis via LHRH release, and diverse downstream effects—supported by a growing body of peer‑reviewed literature published in Nature Communications, Journal of Neuroendocrinology, and other authoritative journals—makes it a valuable addition to any laboratory investigating reproductive endocrinology, neuroendocrine signalling, metastasis suppression, or bone homeostasis. By choosing high‑purity, lab‑verified Kisspeptin-10 (10mg) research peptide from Wuhan Top Miracle Peptides, you ensure that your research is built on a foundation of quality, reproducibility, and scientific integrity.

Ready to advance your research with Kisspeptin-10 (10mg)? Browse our product catalogue, request a wholesale quote, or contact our support team today to discuss your laboratory’s specific needs. Place your order now and receive GMP‑grade Kisspeptin-10 delivered directly to your facility in the UK, EU, or USA.