Nonapeptide-1 (200mg) Research Peptide: An MC1R Antagonist for Melanogenesis & Receptor Studies

If your research investigates melanocortin receptor signalling, melanogenesis pathways, or the regulation of pigmentation at the molecular level, Nonapeptide-1 (200mg) research peptide offers a precisely defined synthetic tool. Also known as Melanostatine-5, this nine‑amino‑acid peptide is a potent and selective antagonist of the melanocortin‑1 receptor (MC1R). By competitively blocking the binding of α‑melanocyte‑stimulating hormone (α‑MSH) to MC1R, Nonapeptide‑1 effectively inhibits the downstream signalling cascade that drives melanin synthesis. This makes it a valuable research tool for laboratories investigating skin pigmentation, adrenal steroidogenesis, and the molecular mechanisms of melanocortin receptor pharmacology.

At Wuhan Top Miracle Peptides, we supply high‑purity Nonapeptide-1 (200mg) research peptide to research institutions and wholesale buyers across the United Kingdom, France, Germany, Spain, Sweden, Italy, and the USA. Every batch is lyophilised, HPLC‑verified to ≥98% purity, and accompanied by a Certificate of Analysis (COA), ensuring your investigations into melanogenesis, MC1R signalling, and pigmentation research begin with uncompromising quality.

What Is Nonapeptide‑1 and How Does It Work?

To fully appreciate the research potential of Nonapeptide-1 (200mg) research peptide, it is essential to understand its biochemical design and the precise mechanism by which it modulates melanocortin receptor signalling.

The Nine‑Amino‑Acid Peptide

Nonapeptide‑1 is a synthetic linear peptide consisting of nine amino acids with the sequence Met‑Pro‑D‑Phe‑Arg‑D‑Trp‑Phe‑Lys‑Pro‑Val‑NH₂. Its molecular formula is C₆₁H₈₇N₁₅O₉S, and its molecular weight is approximately 1206.5 g/mol. It has a CAS number of 158563‑45‑2. The presence of D‑amino acids (D‑Phe and D‑Trp) in the sequence enhances the peptide’s stability and resistance to enzymatic degradation.

Mechanism of Action: Competitive Antagonism at MC1R

The biological activity of Nonapeptide-1 (200mg) research peptide is rooted in its ability to act as a competitive antagonist at the melanocortin‑1 receptor (MC1R):

• MC1R Selectivity: Nonapeptide‑1 is a selective MC1R antagonist with a binding affinity of Ki = 40 nM. It binds to the MC1 receptor on melanocytes with high specificity, effectively blocking the receptor site.

• Inhibition of α‑MSH Signalling: By competitively binding to MC1R, it prevents α‑melanocyte‑stimulating hormone (α‑MSH) from activating the receptor. This blocks the subsequent increase in intracellular cyclic AMP (cAMP), which is the primary second messenger in the melanogenesis cascade.

• Downregulation of Melanogenic Enzymes: The inhibition of MC1R signalling leads to a downregulation of key melanogenic regulators, including tyrosinase, TRP‑1, TRP‑2, and MITF (microphthalmia‑associated transcription factor). This further suppresses melanin production at the transcriptional level.

• Inhibition of Melanosome Dispersion: Nonapeptide‑1 effectively inhibits α‑MSH‑induced melanosome dispersion in melanocytes, a critical step in the pigmentation process.

Research Potency

The peptide has demonstrated potent inhibitory activity in vitro, with an IC₅₀ of 2.5 nM for inhibiting intracellular cAMP elevation and 11 nM for inhibiting melanosome dispersion induced by α‑MSH. These low nanomolar concentrations highlight its high potency as a research tool.

Key Research Applications for Nonapeptide‑1 (200mg)

Nonapeptide-1 (200mg) research peptide is a versatile tool with applications spanning several areas of dermatological and receptor research.

1. Melanogenesis & Pigmentation Research

The most established application of Nonapeptide‑1 lies in its ability to serve as a molecular probe for studying the melanogenesis pathway. Its mechanism as an MC1R antagonist makes it a valuable tool for:

• Investigating the α‑MSH‑MC1R‑cAMP signalling cascade in melanocytes

• Studying the regulation of melanogenic enzymes (tyrosinase, TRP‑1, TRP‑2) and the transcription factor MITF

• Exploring the molecular mechanisms underlying hyperpigmentation disorders such as melasma and age spots

• Developing and validating in vitro models for screening potential melanogenesis inhibitors

Research suggests that Nonapeptide‑1 may reduce melanin synthesis by approximately one‑third in research systems.

2. MC1R Receptor Pharmacology & Signalling Studies

As a selective MC1R antagonist, Nonapeptide‑1 is a valuable tool for:

• Dissecting the structural and functional properties of the melanocortin‑1 receptor

• Studying the kinetics of receptor‑ligand interactions and competitive binding

• Exploring the role of MC1R in melanocyte biology and pigmentation

• Investigating the downstream signalling pathways activated by MC1R

Nonapeptide‑1’s high selectivity (Ki = 40 nM) and potency make it an ideal reference standard for benchmarking novel MC1R‑targeted compounds.

3. Adrenal Steroidogenesis Research

Beyond its role in pigmentation, Nonapeptide‑1 has been studied for its effects on steroid production in the adrenal gland. The peptide can be used to investigate:

• The role of MC1R and related melanocortin receptors in adrenal function

• The regulation of steroidogenesis and its modulation by peptide antagonists

• The interplay between melanocortin receptor signalling and adrenal hormone production

4. Skin Cancer Research

Nonapeptide‑1 has been explored in the context of skin cancer research. Research applications include:

• Investigating the role of MC1R signalling in melanoma biology

• Studying the effects of MC1R antagonism on melanocyte proliferation and transformation

• Exploring potential pathways for modulating pigmentation‑related skin conditions

5. Formulation & Nanotechnology Research

Nonapeptide‑1 has been investigated in advanced delivery systems. For example, a study explored Nonapeptide‑1‑conjugated mesoporous silica nanoparticles (EGCG@NP‑MSN) to improve the stability and bioavailability of EGCG for reversing skin photoaging. This positions the peptide as a valuable tool for:

• Studying peptide‑based targeting and delivery systems

• Investigating the stability and formulation of bioactive peptides

• Developing novel nanoparticle platforms for dermatological research

Chemical & Physical Specifications for Nonapeptide-1 (200mg)

Accurate characterisation of Nonapeptide-1 (200mg) research peptide is essential for reproducible experimental design.

Note: The 200 mg mass refers to the weight of the peptide in its lyophilised form. Nonapeptide‑1 is typically supplied as an acetate salt, which significantly enhances its aqueous solubility (≥50 mg/mL in water).

Reconstitution & Handling Protocol for Nonapeptide‑1 (200mg)

Proper reconstitution of Nonapeptide-1 (200mg) research peptide is critical for maintaining its structural integrity and biological activity.

Step 1 – Preparation

• Bring the sealed Nonapeptide‑1 vial and your chosen diluent (sterile bacteriostatic water or sterile PBS, pH 5‑7) to room temperature. Condensation can degrade the lyophilised powder.

• Clean your work surface with 70% ethanol and lay out sterile syringes, alcohol wipes and a clean work mat.

Step 2 – Vial Sanitisation

• Wipe the rubber stopper of the Nonapeptide‑1 vial with a fresh 70% ethanol wipe. Allow the alcohol to evaporate completely before piercing.

Step 3 – Reconstitution

• Draw your desired volume of diluent into a sterile syringe. A common starting concentration for research applications is 200 mg per 20 mL of diluent, yielding a 10 mg/mL stock solution.

• Insert the needle and slowly inject the diluent against the inner glass wall. Do not spray directly onto the lyophilised powder.

• Gently swirl the vial until the solution is clear and fully dissolved. Do not shake vigorously, as shaking can cause denaturation.

Step 4 – Aliquoting & Storage

• Immediately after dissolution, aliquot the reconstituted Nonapeptide‑1 into single‑use sterile vials.

• Store aliquots at –20°C. Avoid repeated freeze‑thaw cycles.

• Once thawed, store at 2‑8°C and use within 28 days.

Concentration Reference Table for Nonapeptide-1 (200mg)

Adjust diluent volume based on your experimental requirements. Nonapeptide‑1 is fully soluble in water at these concentrations.

Storage & Stability Guidelines for Nonapeptide-1 (200mg)

Why Choose Wuhan Top Miracle Peptides for Nonapeptide‑1 (200mg)?

As an authentic licensed peptide supplier based in the United Kingdom, we supply Nonapeptide-1 (200mg) research peptide and a wide range of research‑grade compounds under rigorous quality assurance protocols, fully aligned with Google’s E‑E‑A‑T standards (Experience, Expertise, Authoritativeness, Trustworthiness).

• Verified Purity: Independent HPLC analysis with purity guaranteed ≥98% for every batch.

• GMP‑Compliant Manufacturing: All peptides are synthesised in ISO 13485:2016 and GMP‑certified facilities, ensuring batch‑to‑batch consistency.

• Full Traceability: Batch‑specific Certificates of Analysis (COA) are available for every order.

• Wholesale Supply: We serve research institutions, laboratories, and wholesale buyers across the UK, EU, and USA with flexible volume pricing and reliable logistics.

• UK‑Based Regulatory Compliance: All products are supplied strictly as research‑grade chemicals for laboratory use only (RUO).

Researchers across the United Kingdom—including those at leading institutions in London, Cambridge, Oxford, Manchester, and Edinburgh—trust us for fast domestic delivery, transparent documentation and professional technical support.

FAQ: Nonapeptide‑1 (200mg) Research Peptide

 What is Nonapeptide‑1 (200mg) research peptide?
Nonapeptide‑1 (Melanostatine‑5) is a synthetic nine‑amino‑acid peptide that acts as a selective antagonist of the melanocortin‑1 receptor (MC1R). It competitively blocks α‑MSH binding, inhibiting cAMP elevation, melanosome dispersion, and melanin synthesis.

What is the sequence and molecular weight of Nonapeptide‑1?
The sequence is Met‑Pro‑D‑Phe‑Arg‑D‑Trp‑Phe‑Lys‑Pro‑Val‑NH₂. Its molecular formula is C₆₁H₈₇N₁₅O₉S, and its molecular weight is approximately 1206.5 g/mol.

What are the primary research applications for Nonapeptide‑1?
Nonapeptide‑1 is used in melanogenesis and pigmentation research, MC1R receptor pharmacology studies, adrenal steroidogenesis research, skin cancer research, and formulation/nanotechnology research.

What is the mechanism of action of Nonapeptide‑1?
Nonapeptide‑1 acts as a competitive antagonist at the MC1R (Ki = 40 nM). It blocks α‑MSH‑induced cAMP elevation (IC₅₀ = 2.5 nM) and melanosome dispersion (IC₅₀ = 11 nM), and downregulates tyrosinase, TRP‑1, TRP‑2, and MITF.

What purity does your Nonapeptide‑1 (200mg) meet?
Our Nonapeptide‑1 is tested to ≥98% purity by HPLC. A Certificate of Analysis (COA) is supplied with every order.

How should I reconstitute Nonapeptide‑1 (200mg)?
Reconstitute with sterile bacteriostatic water or sterile PBS (pH 5‑7). Allow the vial to reach room temperature, then slowly inject the diluent against the inner glass wall and swirl gently. Do not shake.

How should I store reconstituted Nonapeptide‑1?
Reconstituted Nonapeptide‑1 should be stored at 2‑8°C and used within 28 days. For longer storage, aliquot immediately into single‑use vials and store at –20°C for up to 3 months. Avoid repeated freeze‑thaw cycles.

Is Nonapeptide‑1 research peptide legal to purchase in the UK?
Yes. Nonapeptide‑1 is a laboratory reagent sold for research use only (RUO). It is fully legal to purchase, possess, and use in the United Kingdom for legitimate in‑vitro or in‑vivo scientific research, provided it is not marketed with therapeutic claims.

Does Wuhan Top Miracle Peptides ship internationally?
Yes. We ship across the EU (France, Germany, Spain, Sweden, Italy) and to the USA from our UK warehouse. Wholesale pricing is available for bulk orders.

Wholesale Nonapeptide-1 200mg Supply Across the UK and Europe

Wuhan Top Miracle Peptides supports wholesale peptide and cosmetic ingredient buyers in the United Kingdom and international markets.

We Support Enquiries From:

• United Kingdom
• France
• Germany
• Spain
• Sweden
• Italy
• USA
• Wider Europe

For larger Nonapeptide-1 200mg enquiries, contact our team before ordering. This helps confirm stock, wholesale pricing, documentation, shipping options, and buyer requirements.

Where to Buy Nonapeptide-1 200mg in the UK

If you are searching for where to buy Nonapeptide-1 200mg in the UK, Wuhan Top Miracle Peptides provides a simple research-focused enquiry process.

How to Order

1. Review the Nonapeptide-1 200mg product details.
2. Check the current label and batch information.
3. Confirm the INCI name or product identity from documentation.
4. Ask about COA and purity-related documentation.
5. Confirm your shipping country.
6. Discuss wholesale quantity requirements.
7. Place your enquiry with support from our team.

 

Bulk Peptides Page
Shipping & Returns Page
Contact Us Page
Shop Page
Quality / Compliance Page
Google Search Central
GOV.UK / MHRA
ASA / CAP Code Section 12
Scientific or ingredient reference source such as PubMed, NCBI, PubChem, CosIng, or an official cosmetic ingredient database

From its design as a selective MC1R antagonist to its well‑characterised role in inhibiting α‑MSH signalling, cAMP elevation, and melanin synthesis, Nonapeptide-1 (200mg) research peptide provides a precisely defined platform for advanced dermatological and receptor research. Its ability to modulate the melanogenesis pathway through competitive receptor antagonism—supported by high‑affinity binding (Ki = 40 nM), potent in vitro activity (IC₅₀ = 2.5 nM for cAMP inhibition), and documented downregulation of key melanogenic enzymes—makes it a valuable addition to any laboratory investigating pigmentation, MC1R pharmacology, or related signalling pathways. By choosing high‑purity, lab‑verified Nonapeptide-1 (200mg) research peptide from Wuhan Top Miracle Peptides, you ensure that your research is built on a foundation of quality, reproducibility, and scientific integrity.

Ready to advance your research with Nonapeptide‑1 (200mg)? Browse our product catalogue, request a wholesale quote, or contact our support team today to discuss your laboratory’s specific needs. Place your order now and receive GMP‑grade Nonapeptide‑1 delivered directly to your facility in the UK, EU, or USA.